苯磺酸氨氯地平抑制高糖诱导的H9C2细胞凋亡研究

发布时间：2017-12-28 02:14

  本文关键词：苯磺酸氨氯地平抑制高糖诱导的H9C2细胞凋亡研究　出处：《重庆医学》2016年31期 　论文类型：期刊论文

  更多相关文章： 糖尿病 妊娠 心肌细胞肥大 苯磺酸氨氯地平

【摘要】：目的利用细胞实验探讨高糖培养对H9C2细胞凋亡的影响,并探讨苯磺酸氨氯地平的保护作用。方法体外培养大鼠心肌细胞H9C2,分为5mmol/L糖培养组(G1)、25mmol/L糖培养组(G2)、50mmol/L糖培养组(G3)和25mmol/L糖培养组加钙离子通道抑制剂络活喜保护组(G2+N)、50mmol/L糖培养组加络活喜保护组(G3+N)5组,每组分设48h(a)、72h(b)培养两个亚组共10组。AnnexinV/PI结合流式细胞仪检测细胞凋亡率,荧光染色观察[Ca2+]i。结果细胞凋亡率随着高糖刺激时间和高糖浓度的增加而逐渐增高,加络活喜组细胞的凋亡率显著降低(P0.05)。G2、G3组单细胞平均[Ca2+]i活性测定荧光值均较G1组升高(P0.05);G2+N、G3+N组单细胞平均[Ca2+]i活性测定荧光值分别较G2、G3组降低(P0.05)。各a、b亚组间单细胞平均[Ca2+]i活性测定荧光值差异无统计学意义(P0.05)。结论高糖培养H9C2细胞可增加[Ca2+]i从而导致细胞凋亡,苯磺酸氨氯地平可抑制Ca2+内流从而抑制细胞凋亡。
[Abstract]:Objective to investigate the effect of high glucose on the apoptosis of H9C2 cells and to explore the protective effect of amlodipine sulfonate. The myocardial cells of H9C2 rats were cultured in vitro, divided into 5mmol/L culture group (G1), sugar sugar 25mmol/L culture group (G2), 50mmol/L group (G3) and glucose 25mmol/L glucose group plus calcium channel inhibitor (G2+N), amlodipine group 50mmol/L group and amlodipine sugar culture protection group (G3+N) 5 each group is divided into group 48h (a), 72h (b) training of two sub group 10 group. The apoptosis rate was detected by AnnexinV/PI combined with flow cytometry, and [Ca2+]i was observed by fluorescence staining. Results the apoptosis rate increased with high glucose time and high sugar concentration gradually increased, apoptosis rate of cells with amlodipine group decreased significantly (P0.05). The average [Ca2+]i activity of single cells in group G2 and group G3 was higher than that in group G1 (P0.05), and the mean value of [Ca2+]i activity in G2+N group and G3+N group was lower than those in G2 and G3 group (P0.05). There was no significant difference in the fluorescence value of the average [Ca2+]i activity of single cells between the A and B subgroups (P0.05). Conclusion high sugar H9C2 cells can increase [Ca2+]i and lead to cell apoptosis. Amlodipine besylate inhibits Ca2+ influx and inhibits apoptosis.
【作者单位】： 广东省农垦中心医院儿科;广东省农垦中心医院麻醉科;
【基金】：广东省湛江市政资金科技专项竞争性分配项目(2015A01037)
【分类号】：R714.256
【正文快照】： 妊娠期糖尿病(gestational diabetes mellitus,GDM)是临床上最常见的妊娠期间糖代谢异常类型,约占糖尿病孕妇的80%以上[1]。近年GDM呈上升趋势,其对胎儿影响巨大,GDM伴空腹血糖增高者胎儿畸形发生率为6.1%,尤以心血管系统及神经系统畸形最为常见。心脏比神经管更容易受到母体，

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