RNA干扰EFEMP1对卵巢癌细胞增殖、侵袭与转移的影响

发布时间：2018-01-07 01:30

  本文关键词：RNA干扰EFEMP1对卵巢癌细胞增殖、侵袭与转移的影响　出处：《现代妇产科进展》2016年01期 　论文类型：期刊论文

  更多相关文章： EFEMP 卵巢癌 侵袭 转移

【摘要】：目的:探讨fibulin糖蛋白家族中fibulin-3(EFEMP1)对卵巢癌细胞增殖、侵袭与转移的影响,为卵巢癌针对性治疗提供理论基础。方法:利用慢病毒转染RNA干扰的方式,转染具有高侵袭转移能力的S1克隆细胞株,抑制EFEMP1表达。Western blot、Real-time RT-PCR和免疫组化对转染效果进行验证。采用体外细胞功能分析技术检测干扰前后EFEMP1对细胞增殖、侵袭与转移能力的影响。建立裸鼠皮下移植瘤模型,体内观察EFEMP1对肿瘤生长的影响。结果:RNA干扰有效抑制EFEMP1表达,EFEMP1降表达显著抑制肿瘤细胞增殖、侵袭和转移能力,裸鼠体内成瘤率降低并且肿瘤体积显著下降,同时肿瘤组织Fibulin基因表达显著降低。结论:EFEMP1与卵巢癌发生发展及侵袭转移密切相关,为卵巢癌的治疗提供新的思路。
[Abstract]:Objective: to investigate the effect of fibulin-3 (EFEMP1) in fibulin glycoprotein family on the proliferation, invasion and metastasis of ovarian cancer cells. Methods: using lentivirus transfection of RNA interference to transfect S1 clone cell line with high invasion and metastasis ability. Inhibition of EFEMP1 expression. Western blot. Real-time RT-PCR and immunohistochemistry were used to verify the transfection effect. In vitro cell function analysis was used to detect the proliferation of EFEMP1 before and after interference. The effect of EFEMP1 on tumor growth was observed in vivo. Results EFEMP1 interference could effectively inhibit the expression of EFEMP1. The down-regulated expression of EFEMP1 significantly inhibited the proliferation, invasion and metastasis of tumor cells, and the tumorigenesis rate and tumor volume in nude mice decreased significantly. At the same time, the expression of Fibulin gene in tumor tissue was significantly decreased. Conclusion the expression of Fibulin gene is closely related to the occurrence, development, invasion and metastasis of ovarian cancer, which provides a new idea for the treatment of ovarian cancer.
【作者单位】： 山东大学公共卫生学院少儿卫生与妇幼保健系;
【基金】：国家自然基金青年基金(NO:81202056)
【分类号】：R737.31
【正文快照】： 卵巢肿瘤为妇科生殖系统常见肿瘤,死亡率居妇科恶性肿瘤首位,严重危害妇女的身心健康[1-2]。由于缺乏有效的早期诊断和治疗方法,大部分患者确诊时已为晚期,其5年生存率为25%~30%[3]。 近年卵巢癌治疗已取得重大进展,但其死亡率仍高居不下。深入研究发现,肿瘤的浸润与转移构成，

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