宫颈癌组织ITIH5基因启动子及第1外显子甲基化状态与其临床病理特征关系的研究

发布时间：2018-01-13 14:26

本文关键词：宫颈癌组织ITIH5基因启动子及第1外显子甲基化状态与其临床病理特征关系的研究　出处：《现代妇产科进展》2015年04期 　论文类型：期刊论文

【摘要】：目的:探讨宫颈癌组织中人类间胰蛋白酶H5重链(ITIH5)基因启动子及第1外显子区甲基化状态对ITIH5表达的影响,分析ITIH5基因甲基化程度与临床病理特征的关系。方法:选取50例宫颈癌组织和20例正常宫颈组织作为研究对象,采用甲基化特异PCR(MSP)和亚硫酸盐基因组测序法(BGS)检测ITIH5基因启动子及第1外显子区甲基化状态,采用实时荧光定量PCR(qRT-PCR)法检测ITIH5 mRNA表达。结果:宫颈癌组织中ITIH5基因甲基化率明显高于正常宫颈组织(χ2=16.104,P=0.000),ITIH5 mRNA表达量明显低于正常宫颈组织(t=3.640,P=0.001)。宫颈癌组织中ITIH5甲基化率与淋巴结转移密切相关(校正χ2=4.562,P=0.029),而与临床分期、组织病理学分级、脉管浸润、深肌层浸润、病理类型之间无显著相关性(P0.05)。结论:ITIH5基因启动子及第1外显子区Cp G岛的高甲基化可能是宫颈癌组织ITIH5基因表达降低甚至沉默的原因。ITIH5基因可能成为宫颈癌的候选抑癌基因,可能与宫颈癌的发生和淋巴结转移有关。
[Abstract]:Objective: to investigate the effect of promoter and exon 1 methylation of human trypsin H5 heavy chain on the expression of ITIH5 in cervical carcinoma. Methods: 50 cases of cervical carcinoma and 20 cases of normal cervical tissues were selected as study objects. The methylation status of promoter and exon 1 of ITIH5 gene was detected by methylation specific PCR and sulfite genome sequencing. The expression of ITIH5 mRNA was detected by real-time fluorescence quantitative PCR qRT-PCR.Results: the methylation rate of ITIH5 gene in cervical carcinoma was significantly higher than that in normal cervix (P < 0.05). 蠂 2: 16.104; The expression of ITIH5 mRNA was significantly lower than that in normal cervical tissue (3.640). The methylation rate of ITIH5 in cervical carcinoma was closely correlated with lymph node metastasis (蠂 ~ 2 = 4.562P = 0.029), but correlated with clinical stage and histopathological grade. Vascular infiltration, deep muscle layer infiltration. There was no significant correlation between pathological types (P0.05). Conclusion\\\. The hypermethylation of G island may be the reason for the decrease or even silencing of ITIH5 gene expression in cervical cancer tissues. ITIH5 gene may be a candidate tumor suppressor gene for cervical cancer. It may be related to the occurrence of cervical cancer and lymph node metastasis.
【作者单位】：青岛大学医学院附属海慈医院妇产科;青岛大学附属医院妇产科;
【基金】：国家自然科学基金资助项目(No:81172480)
【分类号】：R737.33
【正文快照】： 人类间α胰蛋白酶(inter-α-trypsin inhibitor,ITI)是一种血浆蛋白酶抑制剂分子家族[1],在肿瘤的形成过程中起着重要作用,这个蛋白复合体包含了1条轻链和5条同源的重链(由ITIH1-5编***国家自然科学基金资助项目(No:81172480)码)[2],其重链具有独立于轻链的生物学功能[3],且被

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