异种移植制备小鼠子宫平滑肌瘤动物模型的研究

发布时间：2018-01-14 02:03

本文关键词：异种移植制备小鼠子宫平滑肌瘤动物模型的研究　出处：《现代妇产科进展》2015年06期 　论文类型：期刊论文

【摘要】：目的:通过异种移植的方法制备小鼠子宫平滑肌瘤动物模型。方法:经腹部手术将人子宫肌瘤细胞悬液种植到CB-17Scid小鼠子宫肌层,手术后缝合腹部切口。定期记录实验小鼠的体重变化及临床症状变化。种植子宫肌瘤细胞后第4周末,观察小鼠子宫大体形态,比较子宫系数和子宫横径;HE染色光镜观察小鼠子宫平滑肌的病理变化。结果:与正常组相比,模型组小鼠的子宫重量增加,子宫系数增大,宫颈和宫体最大直径增大。HE染色光镜观察显示,与正常组相比,模型组小鼠子宫平滑肌纤维排列紊乱,出现局灶性增生,平滑肌厚度增厚,排列呈卷云状或编织状,界限不清,平滑肌细胞体积增大,细胞核分布密集,呈活跃增生现象,病变与人体子宫肌瘤样增生一致。结论:经腹部手术将人子宫肌瘤细胞悬液种植到CB-17Scid小鼠子宫肌层的方法能够建立子宫平滑肌瘤小鼠模型。
[Abstract]:Objective: to establish a mouse model of uterine leiomyoma by xenotransplantation. Methods: the suspension of human uterine leiomyoma cells was implanted into the myometrium of CB-17Scid mice by abdominal surgery. After operation, abdominal incision was sutured. The changes of body weight and clinical symptoms of experimental mice were recorded regularly. At the end of the 4th week after implantation of uterine leiomyoma cells, the gross shape of uterus was observed, and the uterine coefficient and uterine transverse diameter were compared. Results: compared with the normal group, the uterine weight of the model group increased and the uterine coefficient increased. Compared with the normal group, the uterine smooth muscle fibers of the model group were disordered, focal hyperplasia and thickness of smooth muscle were increased. Arranged in the shape of convoluted cloud or woven shape, the boundary is unclear, the volume of smooth muscle cells is enlarged, the nucleus is densely distributed, and it is active and proliferative. Conclusion: the method of implantation of human uterine leiomyoma cells into the myometrium of CB-17Scid mice by abdominal surgery can establish the model of uterine leiomyoma mice.
【作者单位】：首都医科大学中医药学院;首都医科大学附属北京妇产医院妇幼保健院;
【基金】：国家自然科学基金资助项目(No:81373812,81073096)
【分类号】：R737.33;R-332
【正文快照】： 子宫肌瘤是女性育龄期生殖系统的良性肿瘤[1],可引起子宫异常出血、盆腔压迫症状、疼痛等,影响生育能力[2],是临床上子宫切除的主要原因[3-4]。目前该病的病因和发病机制尚未清楚。子宫肌瘤动物模型的建立处于研究阶段。CB-17Scid小鼠属于严重联合免疫缺陷小鼠,由于缺乏功能性

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