姜黄素对宫颈癌干细胞增殖、聚集及化疗敏感性的影响

发布时间：2018-01-14 04:17

本文关键词：姜黄素对宫颈癌干细胞增殖、聚集及化疗敏感性的影响　出处：《山东医药》2017年37期 　论文类型：期刊论文

【摘要】：目的探讨姜黄素对宫颈癌干细胞增殖、聚集及化疗敏感性的影响。方法用流式细胞术从人宫颈癌细胞株CASKI中分选CD133+的宫颈癌干细胞。将宫颈癌干细胞随机分为七组,CC10组、CC20组、CC40组、CC60组、CC80组及CC100组分别给予终浓度为10、20、40、60、80、100 mg/m L姜黄素进行培养,对照组给予等量溶剂与培养基培养。用MTT法检测各组细胞增殖抑制率。取对照组、CC60组细胞用无血清培养基培养1周,光镜下计数聚集的直径大于100μm的干细胞球个数,流式细胞术检测宫颈癌干细胞比例。取宫颈癌干细胞随机分为BV组、CC组、BV+CC组、对照组四组,分别加入10 mg/m L贝伐单抗、60 mg/m L姜黄素、10 mg/m L BV+60 mg/m L姜黄素、等量溶剂与培养基,培养48 h后采用流式细胞术测算细胞凋亡率。结果 CC10组、CC20组、CC40组、CC60组、CC80组、CC100组细胞增殖抑制率均高于对照组(P均0.05);且随着姜黄素浓度的增加,宫颈癌干细胞增殖抑制率逐渐升高(P均0.05)。CC60组直径大于100μm的干细胞球个数少于对照组(P0.05),干细胞比例低于对照组(P0.05)。BV+CC组细胞凋亡率高于对照组、BV组、CC组(P均0.05),CC组高于对照组、BV组(P均0.05),BV组高于对照组(P均0.05)。结论姜黄素可抑制宫颈癌干细胞增殖,降低其聚集能力,增加其对贝伐单抗的治疗敏感性。
[Abstract]:Objective to investigate the effect of curcumin on the proliferation of cervical cancer stem cells. Methods Cervical cancer stem cells with CD133 were selected from human cervical cancer cell line CASKI by flow cytometry. Cervical cancer stem cells were randomly divided into seven groups (CC10 group). CC20 group, CC40 group, CC60 group, CC80 group and CC100 group were given the final concentration of 10: 20, 40, 6080, respectively. #number0# mg/m L curcumin was cultured, the control group was cultured with the same amount of solvent and culture medium. The inhibition rate of cell proliferation was detected by MTT method. The cells in CC60 group were cultured in serum-free medium for 1 week, and the number of stem cell balls with diameter more than 100 渭 m was counted under light microscope. The proportion of cervical cancer stem cells was detected by flow cytometry. Cervical cancer stem cells were randomly divided into BV group (BV group) and control group (n = 4) with 10 mg/m L bevacizumab. 60 mg/m L curcumin 10 mg/m L BV 60 mg/m L curcumin, equal amount of solvent and medium. The apoptosis rate was measured by flow cytometry after 48 h culture. Results CC10 group CC20 group CC40 group CC60 group and CC80 group. The inhibition rate of cell proliferation in CC100 group was higher than that in control group (P < 0.05). With the increase of curcumin concentration, the proliferation inhibition rate of cervical cancer stem cells increased gradually (P < 0.05). The number of stem cells with diameter more than 100 渭 m in CC60 group was less than that in control group (P 0.05). The percentage of stem cells in CC group was lower than that in control group (P 0.05). The apoptosis rate in CC group was higher than that in control group (P < 0.05). Conclusion curcumin can inhibit the proliferation of cervical cancer stem cells, decrease its aggregation ability and increase its sensitivity to bevacizumab.
【作者单位】：西安630医院;西安交通大学第二附属医院;
【基金】：陕西省自然科学基金资助项目(2016JM8067)
【分类号】：R737.33
【正文快照】： 宫颈癌是发病率仅次于乳腺癌的女性恶性肿瘤,其发病率逐年升高,且发病年龄呈现年轻化趋势[1]。目前宫颈癌新药的研发多侧重于预防。虽然传统放化疗可部分提高宫颈癌患者的生存率、改善患者预后,但仍无法有效控制宫颈癌的复发、转移及治疗耐药[2,3]。贝伐单抗为一种人源单克隆

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