丙戊酸钠联合全反式维甲酸促进子宫颈癌细胞衰老的发生

发布时间：2018-01-16 08:33

本文关键词：丙戊酸钠联合全反式维甲酸促进子宫颈癌细胞衰老的发生　出处：《安徽医科大学学报》2015年02期 　论文类型：期刊论文

更多相关文章： 子宫颈癌 丙戊酸 维甲酸 细胞衰老

【摘要】：目的研究丙戊酸钠(VPA)联合全反式维甲酸(ATRA)诱导子宫颈癌细胞衰老作用,并探讨其分子机制。方法实验分为对照组、VPA组、ATRA组、VPA+ATRA组,采用3 mmol/L VPA、1μmol/L ATRA单独或联合作用于人子宫颈癌He La及Si Ha细胞,对照组仅加溶媒;采用Cell Titer96湟AQueous法检测细胞增殖;β-半乳糖苷酶化学染色法检测细胞衰老;Q-PCR和Western blot法分别检测衰老相关基因P16、P63、h TERT的mRNA和蛋白表达变化。结果 VPA对He La及Si Ha细胞均有生长抑制作用,与ATRA联合抑制效果明显优于单独用药(P0.05);β-半乳糖苷酶染色显示,VPA+ATRA组衰老细胞比例显著增加,与对照组及VPA组、ATRA组比较,差异有统计学意义(P0.05);QPCR和Western blot法结果显示,VPA单独和联合ATRA可上调P16、P63的表达,降低h TERT的表达,且联合用药优于单独用药及对照组(P0.05)。结论 VPA联合ATRA可抑制子宫颈癌细胞增殖,上调P16和P63的表达,下调h TERT的表达,可能是其诱导细胞衰老的分子机制。
[Abstract]:Objective to study the effect of sodium valproate (VPA) combined with ATRA-ATRA on the senescence of cervical cancer cells and its molecular mechanism. In VPA ATRA group, he La and SIHA cells were treated with 3 mmol/L VPA 1 渭 mol/L ATRA alone or in combination. In the control group, only solvent was added; Cell proliferation was detected by Cell Titer96 Huang AQueous assay. Cell senescence was detected by 尾 -galactosidase staining. Q-PCR and Western blot were used to detect the senescence related gene P16 and P63, respectively. Results VPA could inhibit the growth of He-La and SIHA cells. The inhibitory effect of the combination with ATRA was better than that of P0.05 alone. 尾 -galactosidase staining showed that the percentage of senescent cells in VPA ATRA group was significantly higher than that in control group and VPA group (P 0.05). The results of QPCR and Western blot showed that VPA alone and combined with ATRA could up-regulate the expression of p16p63 and decrease the expression of h TERT. Conclusion VPA combined with ATRA can inhibit the proliferation of cervical cancer cells and up-regulate the expression of P16 and P63. The down-regulation of h TERT expression may be the molecular mechanism of cell senescence induced by h TERT.
【作者单位】：安徽医科大学附属省立医院妇产科;安徽医科大学附属省立医院分子医学重点实验室;卫生部中日友好医院妇产科;
【基金】：国家自然科学基金(编号:81372777、81372779、81072127)
【分类号】：R737.33
【正文快照】： 近年来子宫颈癌的发病率有明显上升和年轻化趋势,发病率以每年2%~3%的速度增长[1]。早期2014-11-03接收子宫颈癌患者可采用手术治疗,而晚期患者主要以铂类化疗为主,但毒副作用大,患者易对其产生耐药性,患者生存率大大降低[2-3]。丙戊酸钠(valproateacid,VPA)是一种传统的抗癫

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