经血来源干细胞移植在小鼠卵巢早衰模型中的定位分布

发布时间：2018-01-16 14:19

本文关键词：经血来源干细胞移植在小鼠卵巢早衰模型中的定位分布　出处：《西安交通大学学报(医学版)》2017年06期 　论文类型：期刊论文

【摘要】：目的探讨经血来源干细胞(menstrual-derived stem cells,MenSCs)移植在卵巢早衰(premature ovarian failure,POF)小鼠模型中的分布及生长情况。方法通过腹腔注射顺铂构建POF小鼠模型,90只8周龄雌性C57/BL6小鼠随机分为3组,MenSCs移植组:利用携带绿色荧光蛋白(green fluorescence protein,GFP)基因的慢病毒感染MenSCs进行标记,建模后经尾静脉注射GFP标记的MenSCs悬液(5.0×107个/mL)200μL;POF组:建模后小鼠尾静脉注射等量PBS;空白对照组:正常小鼠不予任何处理仅尾静脉注射等量PBS。观察移植后各组小鼠的一般情况,移植后第10天处死小鼠测定血清FSH和E2浓度、检测卵巢组织学变化、卵泡计数以及检测GFP荧光信号在卵巢内的分布和定位情况。结果建模后小鼠与空白对照组小鼠比较体质量减轻、食欲减退、活动减少,POF组小鼠FSH水平显著增高,E2水平显著降低,卵巢间质纤维化明显,各级卵泡数均显著减少。MenSCs移植组小鼠自移植后体质量逐渐增加,食欲增强,活动增多,移植后第10天FSH浓度显著低于POF组;E2浓度显著高于POF组;原始卵泡、生长卵泡及成熟卵泡数均显著高于POF组,卵巢间质纤维化程度较POF组减轻,但各项指标仍与空白对照组间存在差异。荧光显微镜下可见GFP-MenSCs定位于卵巢间质,未见于卵泡及颗粒细胞,肝脏、肾脏、肺组织未见GFP绿色荧光信号。结论 MenSCs移植可向POF小鼠卵巢归巢,定位于卵巢间质,对卵巢组织结构及内分泌功能起到修复作用。
[Abstract]:Objective to investigate the menstrual-derived stem cells derived from blood derived stem cells. Mens failure was transplanted into premature ovarian failure of premature ovarian failure. Methods 90 female C57 / BL6 mice were randomly divided into three groups by intraperitoneal injection of cisplatin. MenSCs group: green fluorescence protein was used to carry green fluorescent protein. The lentivirus infected with GFP gene was labeled with MenSCs, and the MenSCs suspension labeled with GFP was injected into the tail vein with 5. 0 脳 10 ~ (7) / mL ~ (200 渭 L) MenSCs. POF group: mice were injected with the same amount of PBSs via caudal vein after modeling. Blank control group: normal mice were only injected with the same amount of PBSs via caudal vein without any treatment. The general situation of each group of mice after transplantation was observed. The mice were killed on the 10th day after transplantation for determination of serum FSH and E2 concentrations. The histological changes of ovary follicle count and the distribution and localization of GFP fluorescence signal in ovary were detected. Results compared with the control group the body mass of the model mice decreased and the appetite decreased. The level of FSH increased significantly and the level of E2 decreased significantly, and the interstitial fibrosis of ovary in the group of decreased activity. The number of follicles of all levels decreased significantly. The body weight increased gradually, the appetite increased and the activity increased after transplantation. The concentration of FSH was significantly lower than that of POF group on the 10th day after transplantation. The concentration of E2 was significantly higher than that of POF group. The number of primordial follicles, growth follicles and mature follicles were significantly higher than that of POF group, and the degree of ovarian interstitial fibrosis was less than that of POF group. GFP-MenSCs was located in the ovary stroma under fluorescence microscope, but not in follicle and granulosa cells, liver and kidney. Conclusion MenSCs transplantation can homing the ovary of POF mice and is located in the stroma of ovary, which can repair the structure and endocrine function of ovary.
【作者单位】：西安交通大学第一附属医院妇产科;陕西省妇幼保健院;西安交通大学第一附属医院麻醉科;
【基金】：国家自然科学基金资助项目(No.81571393)~~
【分类号】：R-332;R711.75
【正文快照】： 卵巢早衰(premature ovarian failure,POF)是较为常见的妇科内分泌疾病,以女性青春期之后,40岁之前出现4个月以上的持续闭经,血清雌激素水平降低,卵泡刺激素水平升高为特征[1-2]。POF致女性闭经、不孕、性功能低下,同时骨质疏松、高脂血症、心血管疾病等的患病风险也大大增加,

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