microRNA-145的表达抑制子宫颈癌细胞的侵袭和迁移

发布时间：2018-01-16 22:04

本文关键词：microRNA-145的表达抑制子宫颈癌细胞的侵袭和迁移　出处：《吉林大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：观察microRNA-145的表达对子宫颈癌细胞侵袭和迁移能力的影响。方法：将实验室连续传代培养的Hela细胞分为2组：（1）：转染microRNA-145类似物的实验（M）组；（2）转染miR-145模拟物阴性对照的对照（NC）组。肿瘤细胞侵袭试验：将两组细胞加入含有0.1%的FBS的DMEM培养基中饥饿18小时，然后将我们将细胞种在transwell小室上室内，聚碳酸酯膜具有通透性，上室内的细胞可以受下层培养液中的成分的影响，从而研究下层培养液的成分对细胞生长、运动等的影响。用棉签擦去基质胶和上室内的细胞后用结晶紫染色。随机选取视野，观察和拍照，，计算通过聚碳酸酯膜的细胞数。肿瘤迁移实验：在实验组和对照组细胞生长的中央区域用微量枪头划线，细胞的中央部分被去除，然后将无血清培养基加入其中，再放入37度5%CO2培养箱，继续培养。至实验设定的时间（0，6，12，24小时），取出细胞培养板，观察中央划痕区是否有周边细胞生长，以此判断细胞的迁移能力，按0，6，12，24小时取样，拍照。所得数据采用Image-Pro Plus6.0及SPSS15.0软件作统计学处理和分析，比较两组间差异程度是否具有统计学意义。实验结果：转染microRNA-145类似物的实验组的侵袭和迁移能力显著低于miR-145模拟物阴性对照的对照组。miR-145可通过调控一些特定的基因和蛋白来影响肿瘤细胞的侵袭和迁移。
[Abstract]:Objective: To observe the effect of microRNA-145 expression on invasion and migration of cervical cancer cells. Methods: The cultured Hela cells were divided into two groups: group 1: microRNA-145 analogue transfection group. 2) the control group transfected with miR-145 analogue negative control group. Tumor cell invasion test: two groups of cells were fed in DMEM medium containing 0.1% FBS for 18 hours, then we planted the cells in the transwell chamber. The polycarbonate membrane is permeable and the cells in the upper chamber can be affected by the components in the lower culture medium, so as to study the cell growth of the lower culture medium. The effect of exercise. The cells were stained with crystal violet with cotton swabs. The number of cells passing through the polycarbonate membrane was calculated by randomly selecting the field of vision, observing and taking pictures, and calculating the number of cells passing through the polycarbonate film. Tumor migration experiment: in the experimental group and control group, the central region of the cell growth was drawn with a trace shot head, the central part of the cell was removed, and then the serum-free medium was added into it. Then put it in 37 掳5 CO _ 2 incubator and continue culture. At the time of experiment, we took out the cell culture plate and observed whether there were peripheral cells growing in the central scratch area. To judge the migration ability of the cells, take samples for 24 hours and take pictures. The data were analyzed by Image-Pro Plus6.0 and SPSS15.0 software. Experimental results: The invasion and migration ability of the experimental group transfected with microRNA-145 analogues was significantly lower than that of the control group with negative miR-145 analog. Genes and proteins affect the invasion and migration of tumor cells.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

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