应用时差成像技术挑选优质胚胎改善单胚胎移植妊娠结局

发布时间：2018-01-17 18:32

  本文关键词：应用时差成像技术挑选优质胚胎改善单胚胎移植妊娠结局　出处：《生殖与避孕》2015年08期 　论文类型：期刊论文

  更多相关文章： 单胚胎移植(SET) 时差成像(TLI) 卵裂模式 时间参数

【摘要】：目的:探讨应用时差成像(TLI)技术基于胚胎卵裂模式及时间参数进行胚胎挑选,对分裂期胚胎单胚胎移植(SET)妊娠结局的影响。方法:1将206个常规IVF周期患者胚胎行TLI动态观察,对其2 349枚非移植胚胎进行囊胚培养,回顾性分析胚胎发育的卵裂模式和时间参数,以囊胚质量为结局进行Logistic回归分析,建立优质胚胎筛选标准。2应用建立的优质胚胎筛选标准开展分裂期胚胎SET的前瞻性研究,用TLI观察88例常规IVF周期患者的胚胎发育情况,挑选优质胚胎进行SET,观察妊娠结局。结果:1分裂方式为正常卵裂、不均卵裂、非轴性卵裂的胚胎具有较高的优质囊胚形成率(分别为57.09%,44.44%及40.91%),时间参数从3-细胞分裂到4-细胞持续的时间(s2)(≤0.50 h)和发育至5-细胞的时间(t5)(≤48.93 h)对优质囊胚形成最具预测价值。2由此建立基于胚胎卵裂模式与时间参数的分层筛选模式,将其应用于分裂期胚胎SET,符合筛选条件的SET组临床妊娠率为73.24%(52/71)。结论:应用TLI,基于胚胎卵裂模式和时间参数建立了分层筛选模式,所挑选的优质胚胎可显著改善分裂期胚胎SET的妊娠结局。
[Abstract]:Objective: to investigate the application of time-difference imaging (TLI) technique in embryo selection based on embryo cleavage model and time parameters. Methods one hundred and twenty six embryos in normal IVF cycle were observed dynamically by TLI. The blastocysts of 2 349 non-transplanted embryos were cultured. The cleavage pattern and time parameters of embryo development were analyzed retrospectively. The Logistic regression analysis was carried out with the result of blastocyst quality. A prospective study on SET of mitotic embryos was carried out by using the established criteria of high quality embryo screening. 88 cases of normal IVF cycle patients were observed by TLI. Results the cleavage pattern of 1: 1 was normal cleavage and uneven cleavage. The embryos with non-axial cleavage had higher rate of blastocyst formation (57.09%, respectively). 44.44% and 40.91). Time parameter from 3- cell division to 4- cell duration (鈮，

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