辅助生殖技术促排卵周期中无特异性病变宫腔积液对精子和胚胎发育的影响

发布时间：2018-01-18 08:37

本文关键词：辅助生殖技术促排卵周期中无特异性病变宫腔积液对精子和胚胎发育的影响　出处：《山东医药》2015年30期 　论文类型：期刊论文

【摘要】：目的观察辅助生殖技术促排卵周期中的无特异性病变宫腔积液(简称宫腔积液)对人高活力精子和小鼠胚胎发育潜能的影响。方法观察组将宫腔积液分别与精子培养液和胚胎培养液按体积浓度分别配制成25%、50%、75%、100%,对照组为单纯精子或胚胎培养液,在相应培养液中分别加入高活力人类精子和2细胞小鼠胚胎;观察接触宫腔积液后精子前向运动率和存活率,以及小鼠胚胎培养后囊胚形成率和孵出囊胚形成率。结果与对照组相比,观察组精子前向运动率、存活率及小鼠囊胚形成率、孵出率明显降低(P均0.05),且随宫腔积液浓度增加降低更明显(P均0.05)。结论宫腔积液可降低人高活力精子的运动和受精能力,同时降低胚胎发育潜能。
[Abstract]:Objective to observe the nonspecific pathological changes of uterine effusion in ovulatory cycle with assisted reproductive technique (abbreviated as uterine effusion). Methods the uterine cavity fluid, sperm culture medium and embryo culture medium were prepared into 25% in the observation group according to the volume concentration of the human high motility sperm and mouse embryo development potential. The control group was pure spermatozoa or embryo culture medium, and the high motility human sperm and 2 cell mouse embryos were added into the corresponding culture medium. The forward motility rate and survival rate of spermatozoa, blastocyst formation rate and hatched blastocyst formation rate after mouse embryo culture were observed. Results compared with the control group, the sperm forward motility rate in the observation group was higher than that in the control group. Survival rate, blastocyst formation rate and hatching rate decreased significantly (0.05). With the increase of the concentration of fluid in uterine cavity, it is more obvious to decrease the concentration of P0.050.Conclusion the effusion of uterine cavity can reduce the mobility and fertilization ability of human highly mobile sperm, and decrease the potential of embryo development at the same time.
【作者单位】：南通妇幼保健院;
【分类号】：R714.8
【正文快照】： 近年来,控制性促排卵在人工授精(IUI)和体外受精—胚胎移植(IVF-ET)等辅助生殖技术中广泛应用,但超生理剂量的激素常产生无特异性病变的宫腔积液(以下简称宫腔积液)。2014年5~12月,我们观察了人精子和小鼠胚胎在不同浓度宫腔积液中的发育情况,探讨宫腔积液对精子和胚胎发育潜

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