5-ALA-PDT联合放疗对常氧与乏氧人卵巢癌SKOV3细胞的杀伤作用

发布时间：2018-01-19 02:09

本文关键词： 放疗光动力疗法 -氨基酮戊酸发光二极管 SKOV细胞　出处：《中国医院药学杂志》2015年15期 　论文类型：期刊论文

【摘要】：目的:探讨5-氨基酮戊酸(5-ALA)介导的LED-光动力疗法(photodynamic therapy,PDT)联合放疗对人卵巢癌SKOV3细胞的杀伤作用。方法:常氧与乏氧下SKOV3细胞各实验组经不同浓度的5-ALA培养4 h后,由不同功率密度的635nm红光和6 Gy X线照射,48 h后MTT法检测细胞存活率。结果:常氧与乏氧下0.1~0.7 mmol·L-1范围内,5-ALA无细胞毒性与放射增敏性(P0.05);5-ALA0.1 mmol·L-1,随着药物浓度与红光功率密度的增加,细胞存活率呈明显的下降趋势;X线与5-ALA-PDT联用,Ra×Rb/Rc比值分别为2.13、1.49,且常氧下联用与顺序无关,乏氧下有关(P0.01)。结论:常氧与乏氧下X线与PDT联用都是协同作用,但乏氧下联用效果弱于常氧,且先X线照射后PDT可能会是一个更好的联用方式。
[Abstract]:Objective: to investigate the photodynamic therapy mediated by 5-ALA-5-aminolevulinic acid. Methods: SKOV3 cells were cultured with 5-ALA for 4 h under normoxic and hypoxic conditions. It was irradiated by 635nm red light and 6Gy X-ray with different power density. Results: the cytotoxicity and radiosensitization of 5-ALA were observed in the range of 0.7 mmol 路L ~ (-1) at 0. 1 mmol 路L ~ (-1) under normoxic and hypoxic conditions. The survival rate of 5-ALA0.1 mmol 路L-1 decreased with the increase of drug concentration and red light power density. The ratio of Ra 脳 Rb/Rc of X-ray and 5-ALA-PDT was 2.13 ~ 1.49, and the combination under normoxic condition was independent of sequence. Conclusion: the combination of X ray and PDT under hypoxia and hypoxia is a synergistic effect, but the effect of combined use under hypoxia is weaker than that under normoxic. And X-ray irradiation after PDT may be a better combination.
【作者单位】：广州军区武汉总医院药剂科;湖北中医药大学药学院;
【分类号】：R737.31
【正文快照】： 据Jemal等[1]报道,2008年全球新增卵巢癌病例225 500例,死亡140 200例。卵巢癌病人的5年生存率仅为20%~30%。临床治疗一般采取手术与化疗为主、放疗为辅的综合治疗方式,但放疗作为辅助治疗尚有不可忽视的地位,孙建衡[2]概括了6种放疗的适应证,其中指明放疗对手术切除残留、隐

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