Th22细胞及相关细胞因子IL-22在人早孕期母胎界面表达的研究

发布时间：2018-01-19 03:34

本文关键词： 母-胎界面正常妊娠早期 Th22细胞 IL-22 流式细胞术酶联免疫吸附法　出处：《广西医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：通过检测正常早孕期妇女外周血、子宫蜕膜局部组织及正常子宫内膜的非孕育龄期妇女外周血、子宫内膜组织中Th22细胞及其特异性效应因子白细胞介素（interleukin，IL）-22的表达，探讨Th22细胞和IL-22在母-胎界面中的作用。方法：选取在广西医科大学第一附属医院自愿行人工流产术的正常妊娠妇女（孕6～10周）15例，子宫内膜正常的非孕生育期妇女13例，收集正常妊娠早期妇女外周血及子宫蜕膜组织、子宫内膜正常的非孕生育期妇女外周血，行流式细胞术检测正常妊娠早期妇女外周血、子宫蜕膜中Th22细胞所占的比例。同时收集正常妊娠早期妇女外周血及子宫蜕膜组织、子宫内膜正常的非孕生育期妇女外周血及子宫内膜，通过ELISA法检测外周血及组织中IL-22水平的变化。结果：1、正常妊娠早期妇女外周血中Th22细胞比例（1.11％±0.24％）比子宫内膜正常的生育期妇女外周血Th22细胞比例（0.94％±0.13％）升高；2、正常妊娠早期妇女母-胎界面中存在Th22细胞，比例为（1.33％±0.32％），且与外周血Th22细胞比例（1.11％±0.24％）相比增加(P＜0.05)。3、正常妊娠早期妇女外周血IL-22表达水平(104.28±4.73)pg/ml,显著高于子宫内膜正常的生育期妇女外周血(84.47±5.99)pg/ml(P＜0.05)。4、与子宫内膜正常的生育期妇女子宫内膜组织IL-22表达水平(88.15±6.49)pg/ml相比，正常妊娠早期妇女蜕膜组织IL-22表达(110.47±6.43)pg/ml，，显著增加(P＜0.05)。5、正常妊娠早期妇女蜕膜组织IL-22水平较外周血增高(P＜0.05)。6、正常妊娠早期妇女外周血、蜕膜组织中Th22细胞百分比与IL-22含量密切正相关（r值分别为0.75，0.93，P0.05）。结论：正常妊娠早期妇女蜕膜局部及外周血Th22细胞、IL-22水平升高可能在母-胎界面的免疫调节中起了作用。
[Abstract]:Objective: to detect the peripheral blood of the normal early pregnant women, the local tissue of decidua and the normal endometrium. Expression of Interleukin-22 in Th22 cells and Interleukin-Interleukin-22 in endometrial tissues. To investigate the role of Th22 cells and IL-22 in maternal-fetal interface. Methods: a total of 15 normal pregnant women (6 weeks of gestation) and 13 cases of non-pregnant women with normal endometrium were selected from the first affiliated Hospital of Guangxi Medical University. The peripheral blood of normal pregnant women and decidua tissues were collected, and the peripheral blood of non-pregnant women with normal endometrium were collected. Flow cytometry was used to detect the peripheral blood of normal women in early pregnancy. The proportion of Th22 cells in decidua. The peripheral blood and decidua tissues of normal pregnant women, and the peripheral blood and endometrium of non-pregnant women with normal endometrium were collected. The changes of IL-22 levels in peripheral blood and tissues were detected by ELISA method. The result is one. The proportion of Th22 cells in peripheral blood of normal pregnant women (1.11% 卤0.24%) was higher than that of women with normal endometrium (0.94% 卤0.13%). Increase; 2. There were Th22 cells in the mother-fetal interface of normal early pregnant women (1.33% 卤0.32%). The proportion of Th22 cells in peripheral blood increased by 1.11% 卤0.24% (P < 0.05). The expression of IL-22 in peripheral blood of normal pregnant women was 104.28 卤4.73 ng / ml. It was significantly higher than that in normal endometrium women (84.47 卤5.99g / ml) (P < 0.05). The expression level of IL-22 was 88.15 卤6.49g / ml in normal endometrium. The expression of IL-22 in decidua of normal early pregnancy women was 110.47 卤6.43 ng / ml, significantly increased (P < 0.05). The level of IL-22 in decidua tissue in early pregnancy was higher than that in peripheral blood (P < 0.05. 6). The positive correlation between the percentage of Th22 cells and the content of IL-22 in decidual tissue was 0.75 ~ 0.93% (P = 0.05), respectively. Conclusion: the increased level of IL-22 in decidua and peripheral Th22 cells may play an important role in the immunomodulation of maternal-fetal interface.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.1

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