雌孕激素受体和p53在卵巢上皮性肿瘤中的表达及临床意义

发布时间：2018-01-24 02:52

  本文关键词： 雌孕激素受体 p53抑癌基因 卵巢上皮癌 免疫组化　出处：《山西医科大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的:本实验运用免疫组织化学技术分析探讨雌激素受体(ER)、孕激素受体(PR)、p53抑癌基因蛋白于上皮性卵巢恶性肿瘤的不同临床期别、不同的病理分级及不同卵巢病理临床类型中的分布情况及表达状态,以此初步探讨雌激素受体(ER)、孕激素受体(PR)及p53蛋白在卵巢上皮恶性肿瘤的发生发展中的临床意义及临床检测价值,为临床治疗卵巢癌提供新思路及基础理论支持。方法:本次试验研究收集标本为来源于山西医科大学附属第一临床医学院妇科2009-2012年有完整的临床随访资料的45例术后病理证实为卵巢上皮性恶性肿瘤患者的常规石蜡标本;运用免疫组织化学的技术定性测定雌激素受体(ER)、孕激素受体(PR)、p53蛋白的阳性表达情况。积极探讨分析雌孕激素受体及p53蛋白与卵巢上皮性肿瘤各种病理组织学类型、不同组织学分级及各个临床期别阶段的关系。结果:1.在所选取的45例上皮性卵巢恶性肿瘤患者病例中,雌激素受体(ER)、孕激素受体(PR)、p53蛋白的阳性表达率分别为55.56%、62.22%、62.2%。2.p53基因蛋白阳性表达提示临床预后较差,与临床分期关系密切,临床分期Ⅲ、Ⅳ期p53基因阳性率表达明显高于Ⅰ、Ⅱ期,即阳性率与临床分期呈正相关,(分别为Ⅰ-Ⅱ期32.14%,Ⅲ-Ⅳ期70.59%,差异有统计学意义)。3.孕激素受体(PR)阳性表达与肿瘤的分化级别及患者的临床分期有着密切的关系。PR在Ⅰ-Ⅱ期的阳性率高于Ⅲ-Ⅳ期,分别为75%、35.29%,差异有统计学意义。在低分化肿瘤中的阳性表达为50%,在高-中分化肿瘤中的阳性表达为84%,差异有统计学意义。结论:雌激素受体(ER)、孕激素受体(PR)及p53蛋白在卵巢上皮性癌中表达较普遍,为常见现象。p53蛋白异常表达的程度与肿瘤的分化级别及患者的临床分期有着密切的关系,研究发现p53阳性表达越高,患者在临床分期中的级别就越晚,生物学行为及临床上患者的病情就会越差。孕激素受体(PR)阳性表达与肿瘤的分化级别及患者的临床分期有着密切的关系。雌激素受体(ER)、孕激素受体(PR)联合p53蛋白的检测对于临床评估卵巢癌患者的预后及指导开展肿瘤内分泌治疗及基因治疗有一定的价值。
[Abstract]:Objective: to investigate the different clinical stages of estrogen receptor ERA, progesterone receptor PPRP p53 tumor suppressor gene protein in epithelial ovarian malignant tumors by immunohistochemical technique. The distribution and expression of estrogen receptor (ERR) were studied in different pathological grades and different clinical types of ovarian pathology. The clinical significance and clinical value of progesterone receptor PRA and p53 protein in the occurrence and development of ovarian epithelial malignant tumor. Methods: to provide new ideas and basic theoretical support for clinical treatment of ovarian cancer. In this study, 45 cases of ovarian epithelial malignancy confirmed by pathology were collected from the first Clinical Medical College affiliated to Shanxi Medical University from 2009 to 2012 with complete clinical follow-up data. Conventional paraffin wax specimens of tumor patients; The estrogen receptor ERA and progesterone receptor PRA were determined by immunohistochemical technique. Positive expression of p53 protein. Positive analysis of estrogen and progesterone receptor p53 protein and ovarian epithelial tumor of various types of histopathology. The relationship between different histological grades and clinical stages. Results 1. Among 45 patients with epithelial ovarian malignant tumor, estrogen receptor ERA and progesterone receptor PRA were selected. The positive expression rates of p53 protein were 55.56 and 62.22, respectively. 2. The positive expression of p53 gene protein suggested that the clinical prognosis was poor and was closely related to clinical stage. The positive rate of p53 gene expression in clinical stage 鈪，

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