上调FOXC1的表达对SKOV3细胞增殖及侵袭的影响

发布时间：2018-01-25 20:35

本文关键词： SKOV细胞 FOXC 增殖侵袭　出处：《郑州大学学报(医学版)》2017年03期 　论文类型：期刊论文

【摘要】：目的:探讨上调FOXC1的表达对卵巢浆液性腺癌SKOV3细胞增殖及侵袭的影响。方法:分别以重组FOXC1慢病毒、慢病毒空载体稳定感染SKOV3细胞(实验组和载体对照组),并以未感染慢病毒的SKOV3细胞为空白对照组,分别应用qRT-PCR和Western blot法检测3组细胞中FOXC1 mRNA和蛋白的表达情况,应用CCK-8法检测3组细胞接种24、48、72 h后的增殖能力,用Transwell小室检测接种24 h细胞的体外侵袭能力。结果:实验组SKOV3细胞FOXC1 mRNA及蛋白的表达均较载体对照组及空白对照组升高(P0.05)。与空白对照组及载体对照组相比,实验组SKOV3细胞感染不同时间的增殖活性均降低,侵袭能力亦降低(P0.05)。结论:上调FOXC1的表达能够抑制SKOV3细胞的增殖及侵袭能力。
[Abstract]:Objective: to investigate the effect of upregulation of FOXC1 on proliferation and invasion of SKOV3 cells in ovarian serous adenocarcinoma. Methods: recombinant FOXC1 lentivirus was used respectively. SKOV3 cells were stably infected with lentivirus empty vector (experimental group and vector control group) and SKOV3 cells without lentivirus infection were used as blank control group. QRT-PCR and Western blot were used to detect the expression of FOXC1 mRNA and protein in the three groups, and CCK-8 method was used to detect the expression of FOXC1 mRNA and protein in the three groups. The proliferative ability of 48? 72h. Transwell chamber was used to detect the invasiveness of SKOV3 cells inoculated for 24 h in vitro. Results: FOXC1 of SKOV3 cells in experimental group. The expression of mRNA and protein were higher than those of vector control group and blank control group. Compared with blank control group and carrier control group. The proliferative activity of SKOV3 cells in the experimental group was decreased at different time points. Conclusion: upregulation of FOXC1 expression can inhibit the proliferation and invasion of SKOV3 cells.
【作者单位】：郑州大学第三附属医院妇产科;
【基金】：河南省科技厅科技攻关项目162102310131
【分类号】：R737.31
【正文快照】： 卵巢上皮性癌(epithelial ovarian cancer,EOC)腺癌是EOC中最常见的病理类型[1]。由于发病隐是女性生殖系统的恶性肿瘤之一,其中卵巢浆液性匿,多数EOC患者就诊时已是晚期,目前在发达国家EOC的病死率居妇科肿瘤之首[2]。因此,了解5'-CTTCTTCCTTGCCTCTCA-3',下游5'-TCCACGAEOC

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