CXCL12-CXCR4生物轴对宫颈癌Hela细胞株的影响

发布时间：2018-01-26 06:27

本文关键词： CXCL CXCR 宫颈癌 Hela细胞　出处：《实用妇产科杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的:探讨CXCR4在宫颈癌细胞株Hela细胞中的表达情况,及CXCL12-CXCR4生物轴对Hela的增殖、迁移和侵袭能力的影响,并研究CXCR4的特异性拮抗剂AMD3100对CXCR4的特异性阻断效果。方法:将宫颈癌Hela细胞株细胞进行体外培养。免疫组化检测Hela细胞中CXCR4的表达情况。采用MTT法研究不同浓度CXCL12对Hela细胞增殖的影响;Transwell法进行实验,判断在无血清培养液的生长条件下,不同浓度的CXCL12和CXCR4拮抗剂AMD3100对Hela细胞迁移、侵袭的影响。结果:CXCR4在Hela细胞的细胞膜、细胞质中有表达。1组Hela细胞的增殖数与2组及3组两两比较,差异有统计学意义(P0.05),4组Hela细胞的增殖数较2组、3组低,差异有统计学意义(P0.05)。与A组Hela细胞的迁移数和侵袭数相比,B组明显更高(P0.05);而C组Hela细胞的迁移数和侵袭数明显高于A组和B组;D组Hela细胞的迁移数和侵袭数均低于A、B、C3组,差异有统计学意义(P0.05)。结论:CXCR4在Hela细胞的细胞膜、细胞质中均表达。CXCL12-CXCR4生物轴可促进宫颈癌Hela细胞株的迁移及侵袭,而CXCR4拮抗剂AMD3100能抑制这一过程,CXCL12-CXCR4生物轴在宫颈癌Hela细胞生长和转移过程中可能起着重要作用。
[Abstract]:Objective: to investigate the expression of CXCR4 in cervical cancer cell line Hela and the effect of CXCL12-CXCR4 axis on the proliferation, migration and invasion of Hela. The specific blocking effect of AMD3100, a specific antagonist of CXCR4, on CXCR4 was studied. Hela cells of cervical cancer were cultured in vitro. The expression of CXCR4 in Hela cells was detected by immunohistochemistry. The effect of different concentration of CXCL12 on Hela cells was studied by MTT method. The effects of colonization; Transwell assay was used to determine the migration of Hela cells in different concentrations of CXCL12 and CXCR4 antagonist AMD3100 under the condition of serum-free medium. Results the proliferation of Hela cells in the cell membrane and cytoplasm of Hela cells was compared with that of group 2 and group 3. The proliferative number of Hela cells in P0. 05 group was lower than that in group 2 and group 3. Compared with group A, the migration and invasion of Hela cells in group B were significantly higher than those in group B (P 0.05). The number of migration and invasion of Hela cells in group C was significantly higher than that in group A and group B. The migration and invasion of Hela cells in group D were lower than those in group C (P 0.05). The expression of. CXCL12-CXCR4 in cytoplasm can promote the migration and invasion of cervical cancer Hela cell line, but AMD3100 can inhibit this process. CXCL12-CXCR4 axis may play an important role in the growth and metastasis of cervical cancer Hela cells.
【作者单位】：河北医科大学附属人民医院石家庄市第一医院;
【基金】：河北省医学科学研究课题计划项目(编号:20130297)
【分类号】：R737.33
【正文快照】： 宫颈癌是女性最常见恶性肿瘤,近几年发病年龄有所提前。研究表明,趋化因子与其受体结合可以促进多种恶性肿瘤细胞的增殖、迁移及血管新生,与恶性肿瘤的侵袭、转移密切相关[1]。趋化因子CXCL12(cxc chemokine ligand 12)属于CXC类趋化因子家族,趋化因子受体CXCR4(cxc chemokine

【参考文献】