滤泡性辅助T细胞亚型与原因不明复发性流产的关系研究

发布时间：2018-01-27 03:19

本文关键词： 原因不明复发性流产滤泡性辅助T细胞 CXCR CCR 蜕膜流式细胞术　出处：《上海交通大学学报(医学版)》2017年10期 　论文类型：期刊论文

【摘要】：目的·探讨原因不明复发性流产(URSA)的发生与滤泡性辅助T细胞(Tfh)亚型的关系。方法·以28例正常人工流产患者为对照组,28例染色体正常的URSA患者为流产组。分离2组患者外周血及子宫蜕膜组织中单个核细胞,用流式细胞术检测CXCR3~+CCR6~-、CXCR3~-CCR6~-及CXCR3~-CCR6~+3种Tfh细胞亚型水平及B细胞水平。结果·流产组蜕膜组织CXCR3~-CCR6~-Tfh细胞水平显著高于对照组(P=0.015),且与B细胞水平有相关性(R~2=0.779,P=0.025)。2组蜕膜组织中另外2种Tfh细胞亚型水平以及外周血中3种Tfh细胞亚型水平差异均无统计学意义(均P0.05)。结论·早孕妇女蜕膜组织局部CXCR3-CCR6-Tfh细胞亚型数量增多可能与URSA发生有关。
[Abstract]:Objective: to investigate the relationship between the occurrence of recurrent abortion with unknown cause and the subtype of follicular assistant T cell (TfH). Methods: 28 normal abortion patients were taken as control group. The mononuclear cells in peripheral blood and decidua tissues were isolated from 28 URSA patients with normal chromosomes, and CXCR3- CCR6- were detected by flow cytometry. CXCR3ON-CCR6ON- and CXCR3ON-CCR6 ~. Results the levels of CXCR3~-CCR6~-Tfh cells in decidual tissue in abortion group were significantly higher than those in control group (P < 0.05). P0. 015). And there was a correlation with B cell level. There was no significant difference in the levels of the other two Tfh cell subtypes in decidual tissues and the three Tfh cell subtypes in peripheral blood (all P 0.05). Conclusion: the increased number of local CXCR3-CCR6-Tfh cell subtypes in decidual tissue of early pregnant women may be related to the occurrence of URSA.
【作者单位】：上海交通大学医学院附属仁济医院妇产科;
【分类号】：R714.21
【正文快照】： 连续发生3次或3次以上的自然流产者称为复发性URSA的发生存在关联。流产(recurrent spontaneous abortion,RSA)[1],其中有60%~80%的患者因致病原因不详,称为原因不明复发性1对象与方法流产(unexplained recurrent spontaneous abortion,URSA)[2]。近年来,URSA发病率逐渐上升,

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