let-7f-5p在不明原因复发性流产绒毛组织中的表达及其功能研究

发布时间：2018-01-27 16:06

本文关键词： 不明原因复发性流产 let-f-p 凋亡　出处：《南京医科大学学报(自然科学版)》2017年08期 　论文类型：期刊论文

【摘要】：目的 :筛选与不明原因复发性流产(unexplained recurrent spontaneous abortion,URSA)相关的微小RNA(miRNA),探讨其在URSA发生中的作用及其可能机制。方法:通过对URSA绒毛组织进行miRNAs测序,初步筛选差异表达的miRNAs;利用实时荧光定量PCR,验证筛选所得miRNAs表达水平;利用DAVID软件对靶基因进行Pathway分析;利用实时荧光定量PCR,检测p53通路中靶基因及其下游基因的表达水平。结果:let-7f-5p在URSA绒毛组织中表达水平显著上升;let-7f-5p的靶基因富集于p53通路,通路中靶基因MDM-X和其下游靶标促生长基因MDM2水平显著下降,促凋亡基因p53表达水平显著上升。结论:let-7f-5p可能调控p53信号通路中MDM-X/MDM2/p53基因,促进细胞凋亡,最终导致URSA发生发展。
[Abstract]:Objective: to screen unexplained recurrent spontaneous abortion for unexplained recurrent abortion. To explore the role and possible mechanism of URSA in the pathogenesis of URSA. Methods: URSA villi were sequenced by miRNAs sequencing. The differential expression of miRNas was preliminarily screened. The expression level of miRNAs was verified by real-time fluorescence quantitative PCR. The target gene was analyzed by Pathway using DAVID software. The expression level of target gene and its downstream gene in p53 pathway was detected by real-time fluorescence quantitative PCR. Results the expression level of 1: let-7f-5p in URSA chorionic villi was significantly increased. The target gene of let-7f-5p was enriched in p53 pathway, and the level of MDM-X and MDM2 in the pathway was significantly decreased. Conclusion the MDM-X/MDM2/p53 gene in the p53 signaling pathway may be regulated by 1: let-7f-5p to promote apoptosis. Eventually lead to the development of URSA.
【作者单位】：江苏省疾病预防控制中心毒理与功能评价所;南京医科大学附属淮安第一医院产科;
【基金】：[基金项目]江苏省“333高层次人才培养工程”(BRA2015490)
【分类号】：R714.21
【正文快照】： 复发性自然流产(recurrent spontaneous abortion,RSA)是指连续发生2次或2次以上妊娠不足20周,或胎儿体重不足500 g的妊娠丢失。RSA病因非常复杂,包括遗传学异常、子宫解剖异常、内分泌异常、感染因素、免疫学、环境因素等已明确病因,但仍约有50%病因并不明确,因此临床上称

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