阴道炎感染患者不同方法检测病原体的对比分析

发布时间：2018-01-29 05:52

本文关键词： 阴道炎病原体检测　出处：《中华医院感染学杂志》2015年06期 　论文类型：期刊论文

【摘要】：目的探讨阴道炎感染患者不同方法检测病原体结果差异性,以期为临床检测方法的选择提供参考依据。方法收集2013年6-12月300例妇科门诊就诊患者送检的阴道分泌物标本,分别采用直接镜检法、Amsel法、培养法、阴道五联检测试剂盒进行检测,并对检测结果进行比较分析。结果培养法显示300例患者送检标本中282例检出病原体,检出阳性率94.00%,主要感染病原体有加德纳菌、滴虫、假丝酵母菌属;细菌性感染检测阳性率镜检法为44.67%、Amsel法为58.67%、五联检测试剂盒为61.33%,调查感染检测阳性率镜检法为33.00%、五联检测试剂盒为42.00%,细菌性感染检测感染阳性率镜检法为14.33%、五联检测试剂盒为18.00%;细菌性感染镜检法检测感染阳性率低于Amsel法、五联检测试剂盒、培养法(P0.05),滴虫感染镜检法检测感染阳性率低于五联检测试剂盒、培养法(P0.05),假丝酵母菌属感染镜检法检测阳性率低于培养法(P0.05)。结论阴道炎感染患者以细菌性感染多见,各种感染病原体检测时显微镜镜检阳性率低,五联检测试剂盒检测阳性率与培养法接近,相对成本较高,临床检测阴道分泌物感染病原微生物时,建议先选择显微镜如无法确定感染病原体或检测结果与临床诊断不符时,可进一步选择五联检测试剂盒检测。
[Abstract]:Objective to explore the difference of pathogen detection results in patients with vaginitis infection by different methods. Methods from June to December, 2013, 300 samples of vaginal secretions from gynecological outpatients were collected and examined by direct microscopy. Amsel method, culture method, vaginal five detection kit for detection, and the results were compared and analyzed. Results the culture method showed that there were 282 cases of pathogens detected in the samples of 300 patients. The positive rate was 94.00, and the main pathogens were Gardnebacterium, trichomonas and Candida. The positive rate of bacterial infection detected by microscope was 44.67 and 58.67 by Amsel method, 61.33 by five test kit and 33.00% by microscopic examination. The positive rate of bacterial infection was 14.33 by microscopic examination and 18.00 by fivefold test kit. The positive rate of bacterial infection detected by microscopic examination was lower than that by Amsel method. The positive rate of Trichomonas infection was lower than that of Amsel method. The positive rate of the culture method was lower than that of the culture method. Conclusion the bacterial infection is more common in the patients with vaginitis. The positive rate of microscopes was low, the positive rate of five kits was close to the culture method, the relative cost was high, and the clinical detection of vaginal secretions infected pathogenic microorganisms. It is suggested that if the microscopes are not able to determine the pathogen or if the results of the test do not conform to the clinical diagnosis, we can further select the five-test kit for detection.
【作者单位】：南阳市中心医院检验科;南阳医学高等专科学校基础医学部;
【基金】：河南省教育厅重点基金资助项目(14B350001)
【分类号】：R711.31;R446.19
【正文快照】： 妇女阴道炎是最常见生殖器炎症,阴道检查是确定妇女阴道分泌物是否存在病原体感染的常规检查方法,临床常用方法是进行显微镜下镜检[1]。随着临床检验设备和检验技术的发展,新的检测方法和试剂在临床上开始应用,但是显微镜检测药物价格低廉,仍然在临床广泛应用。为此,2013年6-1

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