妊娠期糖尿病与胎盘PPARg及FATP-3表达水平与新生儿体脂的关系

发布时间：2018-01-29 07:23

本文关键词： 妊娠期糖尿病过氧化物酶体增殖物激活受体γ FATP- 体脂含量　出处：《基因组学与应用生物学》2017年08期 　论文类型：期刊论文

【摘要】：妊娠期糖尿病(gestational diabetes mellitus,GDM)是在妊娠期间发生或首次发现的糖代谢异常,属于糖尿病的一个独立类型,对GDM的形成和发展机理尚未完全明确。本研究试图探讨GDM与胎盘PPARγ水平的关系,以及对新生儿体脂的影响,以进一步阐明GDM的形成机理。我们选取常规产检的孕妇292例并将其分为两组,GDM孕妇86例(GDM组),正常孕妇206例(对照组)。本研究测量了两组新生儿体格特征,采用RT-PCR、Western blotting、凝胶电泳法分别检测胎盘PPAR、FATP-3和CD36蛋白。研究表明,GDM组新生儿总脂肪质量和体脂含量分别为(0.67±0.21)kg和(18.50±3.28)%,明显高于对照组新生儿(p0.05);GDM组胎盘PPAR m RNA及蛋白相对表达量分别为(0.433±0.098)和(0.892±0.112),明显低于对照组胎盘(p0.05);GDM组胎盘FATP-3蛋白相对表达量为(0.563±0.080),明显低于对照组胎盘(p0.05);胎盘PPAR m RNA及蛋白相对表达量与新生儿体脂含量呈负相关(r=-0.573和-0.691,p0.05),与胎盘FATP-3蛋白表达呈正相关(r=0.463,p0.05)。初步认为,GDM产妇胎盘PPAR表达水平降低,其与脂肪酸转运蛋白FATP-3有一定关联,这可能导致新生儿体脂含量升高,值得进一步研究。
[Abstract]:Gestational diabetes mellitusus (GDM) is an abnormality of glucose metabolism that occurs or is first found during pregnancy. This study attempts to explore the relationship between GDM and placental PPAR 纬 level and its effect on neonatal body fat. In order to further elucidate the formation mechanism of GDM, we selected 292 pregnant women with routine birth examination and divided them into two groups: 86 pregnant women with GDM). 206 normal pregnant women (control group). The physical characteristics of newborns in two groups were measured by RT-PCRG Western blotting. The placental PPARN FATP-3 and CD36 protein were detected by gel electrophoresis. The total fat weight and body fat content in GDM group were 0.67 卤0.21kg and 18.50 卤3.28g, respectively, which were significantly higher than those in control group (P 0.05). The relative expressions of PPAR m RNA and protein in placenta of GDM group were 0.433 卤0.098) and 0.892 卤0.112 respectively. It was significantly lower than that in the control group (P 0.05). The relative expression of FATP-3 protein in placenta of GDM group was 0.563 卤0.080, which was significantly lower than that of control group. The relative expression of PPAR m RNA and protein in placenta was negatively correlated with the body fat content of newborn. The expression of PPAR was positively correlated with the expression of FATP-3 protein in placenta. It is associated with fatty acid transporter FATP-3, which may lead to the increase of body fat content in neonates, which is worthy of further study.
【作者单位】：荆州市第一人民医院;
【基金】：湖北省荆州市第一人民医院资助
【分类号】：R714.256
【正文快照】： *通讯作者,cxm0265@163.comExpression Levels of PPARg and FATP-3 in Placenta of GDM and TheirRelationships with Neonatal Body FatCao Xiaoming*The Frst People's Hospital of Jingzhou,Jingzhou,434000*Corresponding author,cxm0265@163.comPPARg及FATP-3表达水平与

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