两种冷冻方法对牛卵巢组织窦前卵泡活性的影响

发布时间：2018-01-31 01:54

本文关键词： 程序化冷冻玻璃化冷冻窦前卵泡活性　出处：《郑州大学学报(医学版)》2017年01期 　论文类型：期刊论文

【摘要】：目的:探讨两种冷冻方法对牛卵巢组织窦前卵泡活性的影响。方法:取新鲜的牛卵巢组织随机分为新鲜对照组、程序化冷冻组和玻璃化冷冻组,分别加入Liberase DH酶消化,然后挑取窦前卵泡并分类计数,用calcein AM以及ethidium homodimer对窦前卵泡进行荧光染色,鉴定窦前卵泡的活性。结果:3组各级卵泡的数量及分布差异无统计学意义(P=0.765);与新鲜对照组(91%)相比,程序化冷冻组和玻璃化冷冻组窦前卵泡中未受损卵泡所占比例(80%和78%)略有降低(P=0.012)。3组中所占比例占优势的始基卵泡中未受损卵泡率差异无统计学意义(P=0.079);与新鲜对照组相比,两冷冻组初级卵泡中未受损卵泡率有所降低(P0.05),但两冷冻组间差异无统计学意义(P0.05)。结论:程序化冷冻法和玻璃化冷冻法均能较好地保存卵巢组织窦前卵泡的活性。
[Abstract]:Objective: to investigate the effect of two freezing methods on the activity of bovine ovarian preantral follicles. Methods: fresh bovine ovarian tissues were randomly divided into three groups: fresh control group, programmed freezing group and vitrification group. After digesting with Liberase DH enzyme, preantral follicles were collected and counted. The preantral follicles were stained with calcein AM and ethidium homodimer. Results there was no significant difference in the number and distribution of follicles in the three groups (P < 0. 765). Compared with fresh control group (91%). The percentage of undamaged follicles in preantral follicles in programmed and vitrified groups was 80% and 78%, respectively. There was no significant difference in the percentage of undamaged follicles in primordial follicles in group 3 (P 0.079). Compared with the fresh control group, the rate of undamaged follicles in the primary follicles in the two freezing groups was lower than that in the fresh control group (P 0.05). But there was no significant difference between the two groups. Conclusion: programmed freezing and vitrification can better preserve the activity of preantral follicles in ovarian tissue.
【作者单位】：郑州大学第三附属医院生殖中心;
【基金】：河南省国际科技合作计划项目152102410060
【分类号】：R714.8
【正文快照】： △女,1962年2月生,博士研究生,主任医师,研究方向:生殖医学,E-mail:wangxl208@126.com差异无统计学意义(P=0.765);与新鲜对照组(91%)相比,程序化冷冻组和玻璃化冷冻组窦前卵泡中未受损卵泡所占比例(80%和78%)略有降低(P=0.012)。3组中所占比例占优势的始基卵泡中未受损卵泡率

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