葡萄糖调节蛋白78在宫颈鳞癌中的表达及其临床意义

发布时间：2018-01-31 13:53

本文关键词： 宫颈鳞癌内质网应激葡萄糖调节蛋白78 免疫组织化学　出处：《山西医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：探讨葡萄糖调节蛋白78（glucose-regulated protein78，GRP78）在宫颈鳞癌中的表达以及与宫颈鳞癌发生、发展的关系。方法：收集四川大学华西临床医学院2011年6月至2013年6月间经手术切除且经病理证实的宫颈鳞癌标本98例、宫颈上皮内瘤变(cervical intraepithelial neoplasis，CIN)30例和正常宫颈组织30例。采用免疫组化SP法检测GRP78在宫颈鳞癌、CIN和正常宫颈组织中的表达情况。并用统计学软件SPSS13.0分析宫颈鳞癌组织中GRP78的表达与其临床病理参数之间的关系。结果：宫颈鳞癌组织中GRP78阳性细胞的比例明显高于CIN和正常宫颈组织(P0．05)。98例宫颈鳞癌组织中GRP78蛋白阳性表达率为80.6％（79/98），30例CIN组织中GRP78阳性表达率为50％(15/30)，30例正常宫颈组织中GRP78阳性表达率为43.3％(13/30)；正常宫颈组与CIN组之间差异无统计学意义（P=0.829），CIN组与宫颈鳞癌组之间差异有统计学意义（P=0.004），正常宫颈组与宫颈鳞癌组之间差异有统计学意义（P0.001）。无淋巴转移的宫颈鳞癌组织中GRP78的阳性表达率为45.3%，伴有淋巴转移的宫颈鳞癌中GRP78的阳性表达率为82.3%。结果显示伴有淋巴结转移的癌组织中GRP78的表达明显高于无淋巴结转移的癌组织，差别具有统计学意义(P＜0.05)，提示GRP78表达增加与淋巴结转移相关。伴HR-HPV感染的宫颈鳞癌组织中GRP78的阳性表达率为78.5%，不伴HR-HPV感染的宫颈鳞癌组织中GRP78的阳性表达率为47.4%，两组比较，差别有统计学意义(P＜0.05)，，表明GRP78的过表达与宫颈鳞癌组织是否伴HR-HPV感染有关，同时GRP78的表达与宫颈鳞癌患者的年龄、临床分期、病理分级无明显相关性(P＞0.05)。结论：GRP78在宫颈鳞癌组织细胞中呈现高表达，而在CIN及正常宫颈组织中呈低表达。GRP78的表达与宫颈鳞癌的淋巴结转移及HR-HPV感染有关，而与患者的年龄、临床分期、病理分级无关。本研究表明GRP78可能参与了宫颈鳞癌发生、发展的过程，可能与肿瘤的恶性细胞增生有关。
[Abstract]:Objective: to investigate the expression of glucose-regulated glucose-regulated GRP78 in cervical squamous cell carcinoma (SCC) and its association with cervical squamous cell carcinoma (SCC). The relationship between development Methods: 98 cases of cervical squamous cell carcinoma (SCC) surgically resected and confirmed by pathology from June 2011 to June 2013 were collected from West China College of Clinical Medicine of Sichuan University. Cervical intraepithelial neoplasia with cervical intraepithelial neoplasis. Immunohistochemical SP method was used to detect GRP78 in cervical squamous cell carcinoma (SCC). The relationship between the expression of GRP78 and clinicopathological parameters in squamous cell carcinoma of cervix was analyzed by SPSS13.0. Results: the proportion of GRP78 positive cells in cervical squamous cell carcinoma was significantly higher than that in CIN and normal cervical tissue (P0.05). The positive expression rate of GRP78 protein in 98 cases of cervical squamous cell carcinoma was 80.679 / 98). The positive expression rate of GRP78 in 30 cases of CIN was 50%. The positive rate of GRP78 in 30 cases of normal cervix was 43.3%. There was no significant difference between normal cervix group and CIN group. There was a significant difference between normal cervical cancer and cervical squamous cell carcinoma. The positive rate of GRP78 in cervical squamous cell carcinoma without lymphatic metastasis was 45.3%. The positive rate of GRP78 expression in cervical squamous cell carcinoma with lymphatic metastasis was 82.3%. The results showed that the expression of GRP78 was significantly higher in carcinoma with lymph node metastasis than in non-lymph node metastasis. The difference was statistically significant (P < 0.05). It was suggested that the increase of GRP78 expression was related to lymph node metastasis, and the positive rate of GRP78 expression in cervical squamous cell carcinoma with HR-HPV infection was 78.5%. The positive expression rate of GRP78 in cervical squamous cell carcinoma without HR-HPV infection was 47.4% (P < 0.05). The results showed that the overexpression of GRP78 was related to whether the cervical squamous cell carcinoma tissues were associated with HR-HPV infection, and the expression of GRP78 was related to the age and clinical stage of patients with cervical squamous cell carcinoma. There was no significant correlation between pathological grade and pathological grade (P > 0.05). ConclusionTwo one GRP78 is highly expressed in squamous cell carcinoma of cervix. However, the low expression of .GRP78 in CIN and normal cervical tissues was related to lymph node metastasis and HR-HPV infection of squamous cell carcinoma of the cervix, but it was related to the age and clinical stage of the patients. This study suggests that GRP78 may be involved in the occurrence and development of squamous cell carcinoma of the cervix and may be related to the malignant cell proliferation of the tumor.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

【参考文献】