硫酸高乌甲素对人宫颈癌Hela细胞增殖、周期与凋亡的影响

发布时间：2018-02-01 10:45

本文关键词： 硫酸高乌甲素 Hela细胞增殖细胞周期凋亡宫颈癌　出处：《中国药学杂志》2017年12期 　论文类型：期刊论文

【摘要】：目的研究硫酸高乌甲素对人宫颈癌Hela细胞增殖、周期和凋亡的影响及其可能作用机制。方法采用细胞计数试剂盒8(CCK-8)法考察硫酸高乌甲素对Hela细胞的增殖抑制作用,并计算其半抑制浓度(IC_(50));运用流式细胞技术,采用碘化丙啶(PI)单标记法和Annexin V-FITC/PI双标记法检测硫酸高乌甲素作用后Hela细胞的细胞周期及细胞凋亡情况。结果硫酸高乌甲素对人宫颈癌Hela细胞的增殖具有抑制作用,且这种抑制作用具有明显的量效关系(r=0.990 5,P0.01),其对Hela细胞的IC_(50)为0.421 mg·mL~(-1)。经硫酸高乌甲素作用后,Hela细胞出现了明显的G_0/G_1期阻滞,且细胞凋亡率随硫酸高乌甲素浓度的增大而逐渐升高。结论硫酸高乌甲素能抑制Hela细胞的生长,可改变细胞周期分布并诱导其凋亡。
[Abstract]:Objective to study the proliferation of human cervical cancer Hela cell line induced by lappaconitine sulfate. Methods the inhibitory effect of aconitine sulfate on the proliferation of Hela cells was investigated by cell count kit (CCK-8). The half inhibitory concentration of ICS was calculated. Flow cytometry was used. Pyridine iodide (Pi). Single labeling and Annexin. V-FITC / Pi double labeling method was used to detect the cell cycle and apoptosis of Hela cells treated with high aconitine sulfate. Results the proliferation of human cervical cancer Hela cells was inhibited by aconitine sulfate. Yes. And this inhibitory effect has a significant dose-response relationship (P 0.01). The ICS _ (50) of Hela cells was 0.421 mg 路mL ~ (-1) 路L ~ (-1). The G _ 0 / G _ 1 phase arrest of Hela cells was observed after the treatment of aconitine sulfate. The apoptosis rate increased with the increase of the concentration of aconitine sulfate. Conclusion it can inhibit the growth of Hela cells, change the cell cycle distribution and induce apoptosis.
【作者单位】：西北师范大学生命科学学院;甘肃特色植物有效成分制品工程技术研究中心;甘肃省医学科学研究院;
【基金】：甘肃省基础研究创新群体计划项目资助(1506RJIA116)
【分类号】：R737.33
【正文快照】： 临床用氢溴酸高乌甲素为乌头属植物高乌头根中提取的总生物碱,经化学分离获得的单酯型降二碳二萜类单体高乌甲素的氢溴酸盐,是我国首创的非成瘾性镇痛药,也是“癌症病人三阶梯止痛疗法”所用药物,具有显著的镇痛、抗炎、局部麻醉等多种药理活性[1-4]。研究表明,高乌甲素与氢溴

【参考文献】