LRIG1在卵巢浆液性囊腺癌细胞对依托泊苷敏感性中的作用研究

发布时间：2018-02-02 16:09

  本文关键词： 卵巢癌 耐药 亮氨酸重复序列免疫球蛋白样结构域基因-　出处：《中国现代医学杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的探讨亮氨酸重复序列免疫球蛋白样结构域基因-1(LRIG1)在卵巢浆液性囊腺癌(SKOV3)细胞株中对依托泊苷敏感性的可能机制。方法噻唑蓝比色法(MMT)检测不同浓度VP16干预下48 h的SKOV3细胞组、siRNA LRIG1转染SKOV3细胞组、SKOV3/VP16细胞组和siRNA LRIG1转染SKOV3/VP16细胞组的增殖。平板克隆检测细胞增殖,流式检测细胞凋亡情况,实时荧光定量聚合酶链反应(q RT-PCR)检测LRIG1mRNA表达。结果半抑制浓度(IC50)分别为62.90、115.49、156.50和195.42μg/L,siRNA LRIG1转染SKOV3、SKOV3/VP16和siRNA LRIG1转染SKOV3/VP16的耐药指数分别为1.8、2.5和3.1。SKOV3、siRNA LRIG1转染SKOV3、SKOV3/VP16和siRNA LRIG1转染SKOV3/VP16细胞组的克隆率(F=39.338,P=0.000),细胞的LRIG1 mRNA(F=63.095,P=0.000)和凋亡细胞(F=230.046,P=0.000)明显不同,与SKOV3比较,siRNA LRIG1转染SKOV3、SKOV3/VP16和siRNA LRIG1转染SKOV3/VP16细胞的克隆率增加(t=0.026、0.0710和0.125,P=0.042、0.000和0.000),细胞的LRIG1 mRNA降低(t=0.130、0.525和0.825,均P=0.000),凋亡细胞减少(t=12.350、35.506和44.412,均P=0.000),与siRNA LRIG1转染SKOV3比较,SKOV3/VP16和siRNA LRIG1转染SKOV3/VP16细胞的克隆率增加(t=0.044和0.099,P=0.001和0.000),LRIG1 mRNA降低(t=0.395和0.695,均P=0.000),凋亡细胞减少(t=23.156和32063,均P0.05),与SKOV3/VP16比较,siRNA LRIG1转染SKOV3/VP16细胞的克隆率增加(t=0.055,P=0.000),细胞的LRIG1 mRNA降低(t=0.300,P=0.000),凋亡细胞减少(t=8.906,P=0.000)。结论 LRIG1 mRNA影响SKOV3细胞对药物的敏感性,沉默LRIG1的耐药细胞可抑制SKOV3细胞凋亡。
[Abstract]:Objective to investigate the role of leucine repeat immunoglobulin-like domain gene (LRIG1) in ovarian serous cystadenocarcinoma (SKOV3). Methods the possible mechanism of sensitivity to etoposide in cell lines. Methods the SKOV3 cells treated with different concentrations of VP16 for 48 h were detected by thiazolyl blue colorimetric assay. SiRNA LRIG1 was transfected into SKOV3 cells. The proliferation of SKOV3/VP16 cells and SKOV3/VP16 cells transfected with siRNA LRIG1 was observed. The proliferation of SKOV3/VP16 cells was detected by plate clone and apoptosis was detected by flow cytometry. The expression of LRIG1mRNA was detected by real-time fluorescence quantitative polymerase chain reaction (Q RT-PCR). Results the semi-inhibitory concentration of IC50 was 62.90 ~ 115.49, respectively. 156.50 and 195.42 渭 g / L siRNA LRIG1 were transfected into SKOV3. The drug resistance index of SKOV3/VP16 transfected with SKOV3/VP16 and siRNA LRIG1 was 1.82.5 and 3.1.SKOV3, respectively. The clone rate of SKOV3/VP16 cells transfected with siRNA LRIG1 and SKOV3 / SKOV3 / VP16 and siRNA LRIG1 was 39.338. The LRIG1 mRNAs of the cells were significantly different from that of the apoptotic cells. Compared with SKOV3, siRNA LRIG1 was transfected into SKOV3. The clone rates of SKOV3/VP16 cells transfected with SKOV3/VP16 and siRNA LRIG1 were increased by 0.026, 0.0710 and 0.125, respectively. The LRIG1 mRNA of the cells decreased from 0.130 to 0.825, both P0. 000). The number of apoptotic cells decreased from 12.350 to 35.506 and 44.412, respectively, compared with that of siRNA LRIG1 transfected SKOV3. The clone rates of SKOV3/VP16 cells transfected with SKOV3/VP16 and siRNA LRIG1 were increased by 0.044 and 0.099 respectively. P0. 001 and 0. 000 LRIG1 mRNA were decreased by 0. 395 and 0. 695, respectively (P 0. 000). Apoptotic cells were decreased by 23.156 and 32063, both P0.05, compared with SKOV3/VP16. The clone rate of SKOV3/VP16 cells transfected with siRNA LRIG1 was increased by 0.055 P0. 000). The LRIG1 mRNA of the cells was decreased by 0.300, and the apoptotic cells were decreased by 8.906. Conclusion LRIG1 mRNA affects the drug sensitivity of SKOV3 cells and silencing LRIG1 resistant cells can inhibit the apoptosis of SKOV3 cells.
【作者单位】： 桂林医学院第二附属医院妇科;桂林医学院附属医院妇科;桂林医学院第二附属医院病理科;桂林医学院附属医院统计室;
【基金】：桂林市科学研究与技术开发计划项目(No:20130120-3)
【分类号】：R737.31
【正文快照】： 当今,卵巢癌的发生率已经呈现出不断上升的1材料与方法趋势,成为危害女性的重要杀手,发病率仅次于子1.1材料和试剂宫颈癌和子宫体癌而列居第3位,卵巢肿瘤中的卵卵巢浆液性囊腺癌(SKOV3)细胞株(北京大学巢上皮癌的死亡为女性各种肿瘤死亡之首,可见卵人民医院),依托泊苷(VP16)(，

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