大鼠外阴阴道假丝酵母菌病模型中局部与全身免疫机制的研究

发布时间：2018-02-04 21:46

本文关键词： 外阴阴道假丝酵母菌病真菌大鼠模型免疫机制　出处：《菌物学报》2017年03期 　论文类型：期刊论文

【摘要】：外阴阴道假丝酵母菌病(VVC)是妇女常见病及多发病,白假丝酵母(白色念珠菌)是其主要致病菌。我们通过在大鼠皮下注射大剂量雌激素建立大鼠的假发情模型,然后在大鼠阴道内接种白假丝酵母(白色念珠菌)SC5314悬液,建立大鼠的外阴阴道假丝酵母菌病模型。在不同时间点检测大鼠血浆中及阴道局部白细胞介素 2(IL2)、甘露糖结合凝集素(MBL)、Toll样受体 4(TLR4)的值,探讨VVC及复发性外阴阴道假丝酵母菌病(RVVC)的免疫发病机制。我们发现大鼠血清中只有TLR4在第2周出现显著性上升,而在阴道灌洗液中,IL2在第3周显著下降,TLR4在第2和第3周显著上升,MBL在第1至第3周均显著下降。由此我们推断在外阴阴道假丝酵母菌发病过程中,阴道局部免疫功能异常可能与VVC和RVVC的发生密切相关。
[Abstract]:Vulvovaginal candidiasis (VVC) is a common and frequent disease in women. Candida albicans (Candida albicans) is the main pathogen of Candida albicans. We established the pseudoestrus model of rats by subcutaneous injection of large doses of estrogen in rats. Then the rat vagina was inoculated with Candida albicans (Candida albicans) SC5314 suspension. The rat model of vulvovaginal candidiasis was established. The plasma and vagina interleukin-2 and mannose binding lectin (MBL) were detected at different time points. Toll like receptor 4 (TLR4). To investigate the immunopathogenesis of VVC and recurrent vulvovaginal candidiasis, we found that only TLR4 in rat serum increased significantly in the 2nd week, but in vaginal lavage fluid. IL2 decreased significantly in the third week. TLR4 increased significantly in the second and third weeks. It was concluded that TLR4 decreased significantly in the first and third weeks, which suggested that TLR4 might play an important role in the pathogenesis of vulvovaginal candida. The abnormal local immune function of vagina may be closely related to the occurrence of VVC and RVVC.
【作者单位】：浙江大学医学院附属妇产科医院;
【基金】：中国十二五国家科技支撑计划重点项目(2012BAI32B08)~~
【分类号】：R-332;R711.3
【正文快照】： 外阴阴道假丝酵母菌病(VVC)是妇科常见病、多发病,大约75%的生育年龄妇女一生中至少有1次发病,其中40% 50%有过2次或以上的发作史,5% 10%的VVC患者可迁延为复发性外阴阴道假丝酵母菌病(RVVC)(Sobel 2007)。白假丝酵母(白色念珠菌)Candida albicans是VVC的主要致病原,属条件

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