孕期和哺乳期大鼠肝脏胆酸和胆酸调控法尼醇X受体、小异二聚体伴侣受体和胆固醇7α-羟化酶的变化

发布时间：2018-02-06 00:40

本文关键词： 孕鼠哺乳鼠肝脏胆酸法尼醇X受体小异二聚体伴侣受体胆固醇α-羟化酶　出处：《重庆医学》2015年30期 　论文类型：期刊论文

【摘要】：目的研究健康SD鼠在妊娠和哺乳期肝脏胆酸代谢基因的变化。方法取孕10、14、19d及产后1、7、14、21d的大鼠肝脏,检测胆酸水平和肝脏法尼醇X受体(FXR)、小异二聚体伴侣受体(SHP)、胆固醇7α-羟化酶(Cyp7α1)的表达。用Western blot法测Cyp7α1的蛋白表达情况。结果孕晚期健康大鼠,肝脏中胆酸及Cyp7α1表达不升高;哺乳期的大鼠,肝脏胆酸增加,FXR表达升高;在孕晚期及哺乳期靶基因SHP表达均高于对照组,且其表达高峰期是第孕19天,其值可达对照组的6倍之多。结论健康大鼠在产后肝脏胆酸合成增加,而孕期减少,可能与Cyp7α1、FXR及SHP表达有关。
[Abstract]:Objective to study the changes of bile acid metabolism genes in the liver of healthy SD rats during pregnancy and lactation. The levels of cholic acid and hepatic FXRX receptor FXRX, small heterodimer chaperone receptor (SHP) were measured. The expression of cholesterol 7 伪 -hydroxylase (Cyp7 伪 1). The expression of Cyp7 伪 1 protein was measured by Western blot method. The expression of cholic acid and Cyp7 伪 1 in liver was not increased. In lactating rats, cholic acid in liver increased the expression of FXR. The expression of target gene SHP in late pregnancy and lactation was higher than that in control group, and the peak expression of target gene was at the 19th day of gestation, which was 6 times higher than that in control group. Conclusion the synthesis of cholic acid in the liver of healthy rats increased after delivery. The decrease in pregnancy may be related to the expression of Cyp7 伪 1 FXR and SHP.
【作者单位】：遵义医学院第三附属医院妇产科;遵义医学院药理教研室暨贵州省基础药理重点实验室;美国堪萨斯大学医学中心;
【基金】：贵州省科技厅国际合作项目[黔科合外G字(2009)70019] 贵州省教育厅研究生教育创新基地项目(023)
【分类号】：R714.255

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