胎盘中血栓素受体通路与子痫前期发病机制的关系

发布时间：2018-02-07 10:54

  本文关键词： 子痫前期 血栓素合酶 血栓素A2 血栓素受体 非吞噬细胞氧化酶1　出处：《福建医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的： 1.研究子痫前期组和正常妊娠组在年龄、终止孕周、孕妇血压、新生儿出生体重、胎儿生长受限等方面的差异； 2.研究子痫前期患者胎盘中血栓素合酶（TXS）、血栓素A2（TXA2）、血栓素受体（TPr）及非吞噬细胞氧化酶1（NOX1）的表达情况，探讨其与子痫前期发病的关系； 3.研究子痫前期患者胎盘TXA2表达与新生儿体重的关系； 4.研究子痫前期患者胎盘TPr通路与NOX1表达的关系。 方法： 1.收集子痫前期患者36例和正常孕妇34例，分析相关临床资料 2.应用免疫组化MaxVisionTM一步法检测各组胎盘组织TXS、TPr、NOX1表达的位置、分布和表达量。 3.应用实时荧光定量PCR（Real-Time PCR）定量检测各组胎盘组织TXS、TPr、NOX1的mRNA表达量。 4. TXB2为TXA2的稳定降解产物，应用酶联免疫吸附法（ELISA）定量检测各组胎盘组织中TXB2的表达量，反映TXA2的表达情况。 结果： 1.两组孕妇临床资料分析结果显示：两组研究对象在年龄上差异无统计学意义（P0.05），但在终止孕周、收缩压、舒张压、平均动脉压和新生儿体重及胎儿生长受限方面差异有统计学意义（P0.01）。 2.免疫组化结果显示： 2.1TXS蛋白主要表达于胎盘组织细胞滋养细胞、合体滋养细胞、少量绒毛血管内皮细胞的胞浆中，，子痫前期组棕色染色明显强于正常妊娠组染色，且差异有统计学意义（P0.01）。 2.2TPr蛋白主要表达于胎盘组织细胞滋养细胞、合体滋养细胞和绒毛血管内皮细胞的胞浆中，子痫前期组棕色染色与正常妊娠组差异无统计学意义（P0.05）。 2.3NOX1蛋白主要表达于胎盘组织细胞滋养细胞、合体滋养细胞、绒毛血管内皮细胞和间质细胞的胞浆中。子痫前期组棕色染色明显强于正常妊娠组染色，且差异有统计学意义（P0.01）。 3. Real-Time PCR结果显示： 3.1子痫前期组胎盘TXS mRNA表达明显强于正常妊娠组，且差异有统计学意义（P0.05）。 3.2子痫前期组胎盘TPr mRNA表达稍强于正常妊娠组，但差异无统计学意义（P0.05）。 3.3子痫前期组胎盘NOX1mRNA表达明显强于正常妊娠组，且差异有统计学意义（P0.01）。 4. ELISA结果显示： 4.1子痫前期组胎盘TXB2的表达量明显高于正常妊组胎盘，且差异有统计学意义（P0.05）。 4.2子痫前期组胎盘TXB2表达量与新生儿体重呈负相关（r=-0.446，P0.01）。 4.3子痫前期组胎盘TXB2表达量和NOX1mRNA表达呈低度正相关（r=0.367，P0.05）。 结论：子痫前期胎盘组织TXS表达升高导致其催化的产物TXA2增加，TXA2通过激活其受体TPr而导致胎盘缺血缺氧，介导胎盘氧化应激，同时TXA2可能通过激活TPr通路而促进NOX1表达增加，导致胎盘产生过量ROS并向母体血液循环释放，参与子痫前期的发病过程。
[Abstract]:Objective:. 1. To study the differences between preeclampsia group and normal pregnancy group in terms of age, termination of gestational week, maternal blood pressure, birth weight of newborn, fetal growth restriction and so on. 2. To study the expression of thromboxane synthase (TXS), thromboxane A2TXA2 (TPr2) and non-phagocytic oxidase 1 (NOX1) in placenta of patients with preeclampsia, and to explore its relationship with preeclampsia. 3. To study the relationship between placental TXA2 expression and neonatal weight in preeclampsia. 4. To study the relationship between placental TPr pathway and NOX1 expression in preeclampsia. Methods:. 1. 36 preeclampsia patients and 34 normal pregnant women were collected and the clinical data were analyzed. 2. The location, distribution and expression of TXS-1 in placenta were detected by immunohistochemical MaxVisionTM one-step method. 3. The expression of mRNA in placenta tissue of each group was detected by real-time fluorescence quantitative PCR(Real-Time. 4. TXB2 was the stable degradation product of TXA2. The expression of TXB2 in placenta of each group was quantitatively detected by enzyme linked immunosorbent assay (Elisa) to reflect the expression of TXA2. Results:. 1. The results of clinical data analysis of two groups of pregnant women showed that there was no significant difference in age between the two groups, but at the termination of gestational week, systolic blood pressure, diastolic blood pressure, There were significant differences in mean arterial pressure, neonatal body weight and fetal growth restriction (P 0.01). 2. The immunohistochemical results showed that:. 2.1TXS protein was mainly expressed in cytotrophoblast, syncytiotrophoblast and a small amount of villi vascular endothelial cells in placenta. The brown staining in preeclampsia group was significantly stronger than that in normal pregnancy group, and the difference was statistically significant (P 0.01). 2.2TPr protein was mainly expressed in cytotrophoblast, syntrophoblast and villi endothelial cell cytoplasm of placenta. There was no significant difference in brown staining between preeclampsia group and normal pregnancy group (P 0.05). 2.3NOX1 protein was mainly expressed in cytoplasm of trophoblast, syncytiotrophoblast, villi vascular endothelial cell and interstitial cell in placenta. Brown staining in preeclampsia group was stronger than that in normal pregnancy group, and the difference was statistically significant (P 0.01). 3.The Real-Time PCR result shows:. 3.1 the expression of TXS mRNA in placenta of preeclampsia group was significantly higher than that of normal pregnancy group, and the difference was statistically significant (P 0.05). 3.2 the expression of TPr mRNA in placenta of preeclampsia group was slightly higher than that of normal pregnancy group, but the difference was not statistically significant (P 0.05). 3.3 placental NOX1mRNA expression in preeclampsia group was significantly higher than that in normal pregnancy group (P 0.01). 4. The ELISA results show that:. 4.1 placental TXB2 expression in preeclampsia group was significantly higher than that in normal pregnancy group (P 0.05). 4.2 the expression of TXB2 in placenta of preeclampsia group was negatively correlated with neonatal body weight. 4.3 the expression of TXB2 and NOX1mRNA in the placenta of preeclampsia group was positively correlated with the expression of NOX1mRNA in the placenta of preeclampsia. Conclusion: the increase of TXS expression in placenta of preeclampsia leads to the increase of its catalytic product TXA2. TXA2 induces placental ischemia and hypoxia by activating its receptor TPr and mediates placental oxidative stress. Meanwhile, TXA2 may promote the expression of NOX1 by activating TPr pathway. The placenta produces excess ROS and releases to the maternal blood, which is involved in preeclampsia.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.244


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