复发性流产病人绒毛组织中FK506结合蛋白52的表达及意义

发布时间：2018-02-09 16:54

本文关键词： 复发性流产 FK结合蛋白绒毛组织实时定量聚合酶链反应免疫组织化学　出处：《中国实用妇科与产科杂志》2015年07期 　论文类型：期刊论文

【摘要】：目的探讨复发性流产(RSA)病人及正常早孕妇女绒毛组织中FK506结合蛋白52(FKBP52)基因和蛋白的表达。方法选取2012年2月至2013年6月广西医科大学第一附属医院妇产科门诊就诊的30例RSA妊娠妇女(复发性流产组)及同期正常早孕妇女30例(对照组),收集其新鲜绒毛组织,分别应用实时定量聚合酶链反应(RT-PCR)及免疫组织化学方法检测两组绒毛组织中FKBP52基因和蛋白的表达。结果 FKBP52在两组妇女的绒毛组织中均有表达,但与对照组相比,复发性流产组凝胶成像系统半定量分析FKBP52 m RNA(1.484±0.198对1.109±0.292,P0.05)及蛋白表达(91.8±5.3对68.2±8.4,P0.01)均明显降低。结论 FKBP52的表达可能在胚胎着床和早期妊娠的维持中起重要作用,其表达降低可能与RSA的发病密切相关。
[Abstract]:Objective to investigate the expression of FK506 binding protein 52 (FKBP52) gene and protein in chorionic villi of patients with recurrent abortion and normal early pregnant women. Methods from February 2012 to June 2013, the outpatient department of gynecology and obstetrics of the first affiliated Hospital of Guangxi Medical University. 30 RSA pregnant women (recurrent abortion group) and 30 normal early pregnant women (control group) collected their fresh villi. The expression of FKBP52 gene and protein in chorionic villi was detected by real-time quantitative polymerase chain reaction (RT-PCR) and immunohistochemistry. Results the expression of FKBP52 was found in the chorionic villi of both groups, but compared with the control group. The semi-quantitative analysis of FKBP52 m RNA(1.484 卤0.198 vs 1.109 卤0.292 P0.05 and protein expression of 91.8 卤5.3 vs 68.2 卤8.4 P0.01 in recurrent abortion group were significantly decreased. Conclusion the expression of FKBP52 may play an important role in embryo implantation and maintenance of early pregnancy. The decrease of its expression may be closely related to the pathogenesis of RSA.
【作者单位】：广西医科大学第一附属医院产前诊断中心;广西医科大学;
【基金】：广西高等学校优秀人才资助计划(20120083)
【分类号】：R714.21

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