人肌母细胞瘤体注射对人宫颈癌细胞裸鼠移植瘤的治疗观察

发布时间：2018-02-12 01:05

本文关键词： 宫颈癌人肌母细胞顺铂动物实验 p　出处：《山东医药》2017年03期 　论文类型：期刊论文

【摘要】：目的观察人肌母细胞瘤体注射治疗人宫颈癌细胞裸鼠移植瘤的效果,并与顺铂进行比较。方法裸鼠45只,皮下注射He La细胞制作宫颈癌细胞移植瘤,随机分为人肌母细胞组、顺铂组、生理盐水组各15只,分别采取人肌母细胞瘤体注射、顺铂腹腔注射、生理盐水瘤体注射,用药后连续观察4周。于用药前后观察裸鼠一般情况,测算裸鼠体质量及肿瘤体积;采用HE染色观察各组肿瘤组织形态及细胞形态;分别采用免疫组化法、Western blotting法检测肿瘤组织中的p53蛋白。结果人肌母细胞组裸鼠用药后体质量较治疗前增高,肿瘤体积较用药前缩小,且用药后肿瘤体积小于其余两组(P均0.05)。人肌母细胞组裸鼠用药后的反应性、精神、饮食状况均优于其余两组。病理检查示生理盐水组细胞异型性明显、肿瘤细胞体积大、细胞核增多;人肌母细胞组可见大量凋亡坏死的肿瘤细胞,大量细胞固缩、细胞核碎裂,肿瘤组织纤维化明显;顺铂组肿瘤细胞固缩、细胞核裂解不明显,肿瘤组织纤维化程度不显著。人肌母细胞组、顺铂组、生理盐水组肿瘤组织中p53蛋白阳性表达率分别为20.0%、33.3%、60.0%,p53蛋白相对表达量分别为0.321±0.054、0.379±0.067、0.534±0.064,三组相比,P均0.05。结论人肌母细胞瘤体注射可减小人宫颈癌细胞裸鼠移植瘤的体积,促使肿瘤细胞凋亡(机制可能与下调p53蛋白表达有关),抑瘤效果优于顺铂。
[Abstract]:Objective to observe the effect of human myoblast tumor injection on human cervical carcinoma xenografts in nude mice, and compare it with cisplatin. Methods 45 nude mice were injected subcutaneously with he La cells to make cervical carcinoma transplanted tumor, and they were randomly divided into human myoblast group. Cisplatin group (n = 15) and saline group (n = 15) were injected with human myoblast, intraperitoneal injection of cisplatin and injection of normal saline respectively. The body mass and tumor volume of nude mice were measured and the morphology of tumor tissues and cells in each group were observed by HE staining. P53 protein in tumor tissue was detected by immunohistochemistry and Western blotting. Results the body mass of nude mice in human myoblast group was higher than that before treatment, and the tumor volume was smaller than that before treatment. The tumor volume was smaller than that of the other two groups (P 0.05). The reactivity, spirit and diet of nude mice in the human myoblast group were better than those in the other two groups. The pathological examination showed that the cells in the saline group were obviously abnormal and the tumor cells were large. Large numbers of apoptotic and necrotic tumor cells were observed in the human myoblast group, with large numbers of cells pyknosis, nuclear fragmentation, and tumor tissue fibrosis, while in the cisplatin group, the tumor cells were pyknotic, and the nucleus cleavage was not obvious. The degree of fibrosis in tumor tissue was not significant. Human myoblast group, cisplatin group, The positive expression rates of p53 protein in normal saline group were 20.0 / 33.3and 0.321 卤0.054 + 0.379 卤0.067 卤0.534 卤0.064, respectively. Conclusion Human myoblast tumor injection can reduce the volume of human cervical carcinoma xenografts in nude mice. The mechanism may be related to the down-regulation of p53 protein expression, and the inhibitory effect is better than cisplatin.
【作者单位】：郑州大学第五附属医院;
【基金】：河南省科技攻关计划普通项目(152102310074)
【分类号】：R737.33

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