纳米雄黄对人宫颈癌细胞增殖和凋亡的影响

发布时间：2018-02-13 10:35

本文关键词： 纳米雄黄宫颈癌凋亡 E E　出处：《中南大学学报(医学版)》2015年10期 　论文类型：期刊论文

【摘要】：目的:探讨纳米雄黄对不同宫颈癌细胞株Hela(HPV18阳性,腺癌),Caski(HPV16阳性,鳞癌),C33A(HP V阴性,腺癌)增殖、凋亡的影响。方法:体外培养3种不同宫颈癌细胞,采用不同质量浓度(5,10,2 0,4 0 mg/L)纳米雄黄作用于不同宫颈癌细胞2 4,4 8,7 2,9 6 h,相差显微镜下观察不同质量浓度组与对照组细胞形态学变化;MTT法测定药物对细胞生长的抑制作用;流式细胞仪检测细胞凋亡及细胞周期。结果:纳米雄黄混悬液(5,10,20,40 mg/L)作用于Caski,Hela,C33A细胞24,48,72,96 h,均可抑制细胞增殖,且呈时间剂量依赖性,抑制率波动于9.02%~49.06%,以20 mg/L质量浓度组为例,抑制率Caski(39.15%)Hela(36.17%)C33A(30.56%)。随着质量浓度的增加,早期凋亡、中晚期凋亡细胞均增加,凋亡率(早期+中晚期凋亡率)波动于19.29%~99.54%,以20 mg/L质量浓度组为例,凋亡率Caski(60.43±2.88)%Hela(41.95±3.01)%C33A(43.49±2.19)%。细胞周期结果显示高质量浓度组(20,40 mg/L)对Hela,Caski细胞有G2/M期阻滞。结论:纳米雄黄混悬液可抑制不同宫颈癌细胞株的增殖,促进细胞凋亡,其对Caski细胞的作用最强,对Hela细胞次之,对C33A细胞相对最弱。对HPV阳性的细胞可产生G2/M期阻滞。
[Abstract]:Objective: to investigate the effects of nano-realgar on the proliferation and apoptosis of different cervical cancer cell lines (Hela(HPV18 positive, Casikius HPV16 positive, squamous cell carcinoma C33AHPV negative, adenocarcinoma). Methods: three kinds of cervical cancer cells were cultured in vitro. Nano realgar was used to treat different cervical cancer cells with different concentration of 1010 mg / L (40 mg / L) for 6 h. The cell morphological changes of different concentration groups and control group were observed under phase contrast microscope and MTT assay was used to determine the inhibitory effect of the drug on cell growth. Cell apoptosis and cell cycle were measured by flow cytometry. Results: the cell proliferation was inhibited in a time and dose-dependent manner when treated with nano-realgar suspension (51010C / L) for 2448C 33A cells for 24 h, and the inhibition rate fluctuated from 9.02 to 49.065.In the 20 mg/L mass concentration group, the cell proliferation was inhibited in a dose-dependent manner, and the inhibition rate varied from 9.02to 49.06mg / L, especially in the 20 mg/L group, and the proliferation of the cells was inhibited in a dose-dependent manner. With the increase of mass concentration, early apoptosis and late apoptotic cells increased, and the apoptotic rate (early, late stage apoptosis rate) fluctuated at 19.29 ~ 99.54%, taking the 20 mg/L mass concentration group as an example. The cell cycle results showed that the high concentration group (20 mg / L) had G 2 / M phase arrest on Hela Caski cells. Conclusion: nano realgar suspension can inhibit the proliferation of different cervical cancer cell lines and promote apoptosis, and its effect on Caski cells is the strongest. For Hela cells, it was the weakest for C33A cells, and G2 / M phase arrest was found for HPV positive cells.
【作者单位】：中南大学湘雅三医院妇产科;
【基金】：湖南省中医药管理局一般项目(201165) 长沙市科技计划项目(K1106039-31)~~
【分类号】：R737.33

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