化疗免疫“窗口期”对卵巢癌治疗影响的实验研究

发布时间：2018-02-13 13:32

本文关键词： 紫杉醇卡铂卵巢癌免疫功能 CTL转输　出处：《现代妇产科进展》2015年04期 　论文类型：期刊论文

【摘要】：目的:采用荷瘤动物模型经化疗联合被动免疫治疗,初步探讨化疗免疫"窗口期"对卵巢癌免疫应答的影响及其可能的免疫学机制。方法:以荷瘤大鼠为研究对象,根据紫杉醇联合卡铂对荷瘤鼠免疫功能改变的不同时期给予CTL(cytotoxic T-lymphocyte)主动免疫治疗。观察荷瘤体积及机体免疫微环境改变。结果:荷瘤鼠紫杉醇和卡铂化疗后不同时期给予CTL转输治疗,其中化疗后第6天即淋巴细胞数最少期,给予免疫治疗,肿瘤生长最缓慢;CT扫描结果示,化疗后6天+免疫治疗组的瘤体积最小,且与其余治疗组有统计学意义。荷瘤鼠免疫功能示,化疗后6天+免疫治疗组中淋巴细胞抗原特异性增殖及CD8+T细胞最明显,而Treg(T-Lymphocytes,Regulatory)细胞明显降低。结论:紫杉醇和卡铂联合CTL治疗肿瘤具有协同作用,其中化疗后6天(即淋巴细胞降到最低期)是CTL免疫治疗的最佳时间点。化疗造成的肿瘤个体免疫功能的低下或是免疫系统空间的释放(所谓的免疫"窗口期")为诱导特异性的抗肿瘤免疫应答提供了可能,免疫状态的检测是保证肿瘤患者化疗联合免疫治疗方案治疗有效性的重要前提之一。
[Abstract]:Objective: to explore the effect of chemotherapy-immunized "window period" on immune response of ovarian cancer and its possible immunological mechanism by chemotherapy combined with passive immunotherapy. According to the effect of paclitaxel combined with carboplatin on immune function changes in tumor-bearing mice, CTL(cytotoxic T-lymphocyte-based active immunotherapy was given. The tumor volume and immune microenvironment were observed. Results: paclitaxel and carboplatin were treated with paclitaxel and carboplatin at different times after chemotherapy. The patients were given CTL transfusions. On the 6th day after chemotherapy, the least number of lymphocytes was given, and the tumor growth was the slowest. The tumor volume was the smallest in the immunotherapy group on the 6th day after chemotherapy. The immunological function of tumor-bearing mice showed that the lymphocyte antigen specific proliferation and CD8 T cells were the most obvious in the immunotherapy group 6 days after chemotherapy. Conclusion: paclitaxel and carboplatin combined with CTL have synergistic effect in the treatment of tumor. The best time point for CTL immunotherapy is 6 days after chemotherapy (i.e. lymphocytes are reduced to the minimum stage). The immune function of tumor individual caused by chemotherapy or the release of immune system space (so-called immune "window period"). It is possible to induce a specific anti-tumor immune response, The detection of immune state is one of the important prerequisites to ensure the effectiveness of chemotherapy combined with immunotherapy in tumor patients.
【作者单位】：山西省人民医院妇产科;上海交通大学附属仁济医院妇产科;
【基金】：国家自然基金项目(No:30801363) 山西省自然基金项目(No:2013011045-5)
【分类号】：R737.31

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