表没食子儿茶素没食子酸酯对人子宫颈癌细胞株HeLa增殖和凋亡的影响

发布时间：2018-02-16 21:05

本文关键词： 表没食子儿茶素没食子酸酯 HeLa 增殖凋亡 p/NF-κB　出处：《现代妇产科进展》2017年01期 　论文类型：期刊论文

【摘要】：目的:探讨表没食子儿茶素没食子酸酯(EGCG)对人宫颈癌细胞株HeLa的作用机制。方法:将对数生长期的HeLa细胞用不同剂量的EGCG(0,10,20,40μmol)处理。细胞培养72h后,MTT法检测细胞增殖,流式细胞术检测细胞凋亡,Western blot法检测细胞磷酸化-丝裂原活化蛋白激酶(p-p38)、丝裂原活化蛋白激酶(p38)、磷酸化核因子-κB(p-NF-κB)、核因子-κB(NF-κB)、B细胞淋巴因子2(Bcl-2)、Bcl-2相关X蛋白(Bax)、活化半胱胺酸蛋白酶蛋白3(Cleaved-Caspase-3)表达。ELISA和RT-PCR法分别检测细胞上清和细胞内肿瘤坏死因子α(TNF-α)、白介素-1β(IL-1β)和白介素-8(IL-8)表达。结果:EGCG可呈剂量依赖性诱导HeLa细胞增殖抑制与凋亡,并可增加Bax和cleavedCaspase3表达,下调p-p38、p-NF-κB、Bcl-2、TNF-α、IL-1β和IL-8表达,而对p38和NF-κB表达无明显影响。结论:EGCG可诱导HeLa细胞增殖抑制和凋亡,其作用机制与抑制p38/NF-κB信号通路介导的炎症相关。
[Abstract]:Aim: to investigate the mechanism of epigallocatechin gallate (EGCG) on human cervical cancer cell line HeLa. Methods: HeLa cells in logarithmic growth phase were treated with different doses of EGCG0A10102040 渭 mol. After 72 hours of culture, cell proliferation was detected by MTT assay. Detection of apoptosis by flow cytometry and Western blot assay for the detection of phosphorylated mitogen-activated protein kinase p-p38, mitogen-activated protein kinase p38, phosphorylated nuclear factor- 魏 Bnp-NF- 魏 B, nuclear factor- 魏 BNF- 魏 BnB cell lymphoid factor 2Bcl-2Bcl-2 related X protein Baxanus, activation of cysteone. The expression of Cleaved-Caspase-3) was detected by Elisa and RT-PCR, respectively. The expression of TNF- 伪 (IL-1 尾) and IL-8IL-8 in supernatant and in cells were detected respectively. Results the proliferation inhibition and apoptosis of HeLa cells were induced in a dose-dependent manner. It also increased the expression of Bax and cleavedCaspase3, down-regulated the expression of p-p38, p-NF- 魏 B, Bcl-2, TNF- 伪, IL-1 尾 and IL-8, but had no obvious effect on the expression of p38 and NF- 魏 B. ConclusionThe expression of p38 and NF- 魏 B could be induced by the inhibition of proliferation and apoptosis of HeLa cells, and the mechanism is related to the inhibition of p38% NF- 魏 B signaling pathway mediated inflammation.
【作者单位】：四川大学华西第四医院重症监护室;
【分类号】：R737.33

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