慢病毒介导的ATF5基因沉默及其对荷人卵巢癌裸鼠移植瘤增殖的影响

发布时间：2018-02-17 00:25

本文关键词： 卵巢肿瘤 RNA干扰慢病毒载体转录激活因子裸鼠　出处：《山东医药》2014年16期 　论文类型：期刊论文

【摘要】：目的观察慢病毒介导的人转录激活因子5(ATF5)基因沉默效果,及其对荷人卵巢癌裸鼠移植瘤增殖的影响。方法建立荷人卵巢癌裸鼠模型30只,随机分为实验组、阴性对照组、空白对照组各10只,分别瘤内注射ATF5-siRNA慢病毒、空载慢病毒、PBS。观察移植瘤生长情况,绘制移植瘤生长曲线。4周后处死裸鼠,取出移植瘤组织并称重,计算实验组抑瘤率。采用RT-PCR和Western blot方法检测各组ATF5 mRNA和蛋白相对水平,TUNEL法检测各组移植瘤组织凋亡指数(AI)。结果实验组移植瘤生长速度明显小于阴性对照组和空白对照组,其抑瘤率为49.60%。实验组ATF5 mRNA及蛋白相对表达量、AI分别为0.40±0.09、0.46±0.05、34.63%±1.91%,阴性对照组分别为0.67±0.07、0.72±0.03、6.04%±0.19%,空白对照组分别为0.80±0.07、0.82±0.05、5.07%±0.20%;实验组与阴性对照组、空白对照组比较,P均0.01;空白对照组与阴性对照组比较,P0.05。结论慢病毒介导的ATF5-siRNA能抑制荷人卵巢癌裸鼠移植瘤中ATF5基因的表达,并明显抑制移植瘤的生长、促进其凋亡。
[Abstract]:Objective to observe the silencing effect of human transcriptional activator 5 (ATF5) gene mediated by lentivirus and its effect on the proliferation of transplanted tumor of human ovarian carcinoma in nude mice. Methods Thirty nude mice bearing human ovarian cancer were randomly divided into experimental group and negative control group. ATF5-siRNA lentivirus was injected into the control group (n = 10). The growth of the transplanted tumor was observed, the growth curve of the transplanted tumor was drawn for 4 weeks, and the transplanted tumor tissue was removed and weighed. RT-PCR and Western blot methods were used to detect ATF5 mRNA and relative protein level Tunel to detect the apoptosis index of transplanted tumor tissue in each group. Results the growth rate of transplanted tumor in the experimental group was significantly lower than that in the negative control group and the blank control group. The inhibition rate of ATF5 mRNA and relative protein expression in the experimental group was 0.40 卤0.09 卤0.05 卤0.46 卤0.05U 34.63% 卤1.91, respectively, in the negative control group was 0.67 卤0.070.72 卤0.03n 6.04% 卤0.19, and in the blank control group was 0.80 卤0.070.82 卤0.055.07% 卤0.20%, respectively. Conclusion Lentivirus-mediated ATF5-siRNA can inhibit the expression of ATF5 gene, inhibit the growth of transplanted tumor and promote apoptosis in nude mice bearing human ovarian carcinoma.
【作者单位】：青岛大学医学院附属医院;青岛大学;
【分类号】：R737.31

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