核因子kappa B1和RelA基因多态性与汉族女性卵巢上皮性癌的关系研究

发布时间：2018-02-19 23:53

本文关键词： 卵巢肿瘤多态性单核苷酸汉族 NF-κB RelA　出处：《中国全科医学》2017年33期 　论文类型：期刊论文

【摘要】：背景炎症与肿瘤关系密切,核因子kappa B(NF-κB)信号通路是重要的炎症通路之一,单核苷酸多态性(SNPs)与疾病易感性亦存在重要关联,p50/p65复合体是NF-κB信号通路中调节靶基因转录的重要分子。目的探讨NF-κB1、RelA基因多态性与汉族女性卵巢上皮性癌的关系,以期从分子水平为卵巢上皮性癌的预防和诊治提供依据。方法 2014年2月—2015年2月选取1999—2010年于北京大学第三医院、北京肿瘤医院、北京医院妇科进行手术治疗的汉族卵巢上皮性癌患者286例为病例组。同期选取与病例组年龄匹配的在上述医院体检健康的汉族女性302例为对照组。提取研究对象DNA,利用Hap Map数据库中中国北京汉族人群(CHB)NF-κB1、RelA基因包含两端2 kb邻近区域的全部SNPs位点的基因分型数据,选择SNPs位点,NF-κB1基因共确定了4个SNPs位点,分别是rs3774934(A/G)、rs3774938(A/G)、rs230510(A/T)、rs909332(A/T);RelA基因共确定了3个SNPs位点,分别是rs10896027(C/G)、rs7119750(C/T)、rs11820062(C/T)。采用Taq Man探针法对SNPs位点进行基因分型,根据基因分型数据,构建单倍域图,推断每例研究对象的单体型、双体型情况。结果对照组rs3774934、rs3774938、rs230510、rs909332、rs10896027、rs7119750、rs11820062均符合Hardy-Weinberg平衡(P0.05)。两组NF-κB1基因rs3774934、rs3774938、rs230510位点基因型和等位基因频率比较,差异均无统计学意义(P0.05);两组NF-κB1基因rs909332位点基因型比较,差异有统计学意义(P0.05);两组NF-κB1基因rs909332位点等位基因频率比较,差异无统计学意义(P0.05)。多因素Logistic回归分析结果显示,NF-κB1基因rs3774934、rs3774938、rs230510、rs909332位点基因型均不是卵巢上皮性癌的影响因素(P0.05);NF-κB1基因单体型均不是卵巢上皮性癌的影响因素(P0.05);NF-κB1基因双体型GA-GA是卵巢上皮性癌的影响因素(P0.05)。两组RelA基因rs10896027、rs7119750、rs11820062位点基因型和等位基因频率比较,差异均无统计学意义(P0.05)。多因素Logistic回归分析结果显示,RelA基因rs10896027、rs7119750、rs11820062位点基因型均不是卵巢上皮性癌的影响因素(P0.05);RelA基因单体型不是卵巢上皮性癌的影响因素(P0.05);RelA基因双体型CC-CC是卵巢上皮性癌的影响因素(P0.05)。结论 NF-κB、RelA基因多态性与汉族女性卵巢上皮性癌易感性具有一定关系,NF-κB1基因双体型——rs3774934(GA)和rs3774938(AG)的GA-GA、RelA基因双体型——rs7119750(CT)和rs11820062(CT)的CC-CC与卵巢上皮性癌的易感性有关。
[Abstract]:Background inflammation is closely related to tumor. Nuclear factor kappa BnNF- 魏 B signal pathway is one of the important inflammatory pathways. Single nucleotide polymorphisms (SNPs) are also associated with disease susceptibility. The p50 / p65 complex is an important molecule in NF- 魏 B signaling pathway that regulates the transcription of target genes. Objective to investigate the relationship between NF- 魏 B1 and RelA gene polymorphism and ovarian epithelial carcinoma in Han women. Methods from February 2014 to February 2015, we selected the third Hospital of Peking University, Beijing Cancer Hospital, from February 2014 to February 2015. 286 patients with ovarian epithelial carcinoma of Han nationality undergoing gynecological surgery in Beijing Hospital were selected as the case group. 302 healthy Han women in the hospital were selected as the control group during the same period. Hap Map database was used to analyze the genotyping data of all SNPs loci at the adjacent region of 2 kb in the Hap Map database of Chinese Han population. Four SNPs loci were identified in the SNPs locus, namely rs3774934A / R / rs3774938A / rs230510A / rs909332A / T / T = 3 SNPs loci were identified respectively as rs10896027Cr / R / rs7119750 / rs11820062CT / rs11820062CT-P, respectively. Based on the genotyping data, three SNPs loci were identified, which were rs10896027Crs7119750t / rs11820062CT / rs11820062CT / rs11820062CT / rs11820062.The Taq Man probe method was used to type the SNPs locus, and based on the genotyping data, a single region map was constructed. Results in the control group, rs3774934, rs3774938, rs230510, rs909332rs10896027rs7119750rs11820062 all accord with the Hardy-Weinberg equilibrium P0.055.The genotype and allele frequency of NF- 魏 B1 gene rs3774934, rs37749338, rs230510 were not significantly different between the two groups (P 0.05). The allelic frequencies of rs909332 loci of NF- 魏 B1 gene were compared between the two groups. The results of multivariate Logistic regression analysis showed that the genotype of rs3774934 / rs3774938 / rs230510rs909332 was not the influencing factor of ovarian epithelial carcinoma. The haplotypes of NF- 魏 B1 gene were not the influencing factors of ovarian epithelial carcinoma. GA-GA is the influential factor of ovarian epithelial carcinoma (P 0.05). The genotypes and allelic frequencies of RelA gene rs10896027rs7119750rs11820062 were compared between the two groups. The results of multivariate Logistic regression analysis showed that the genotype of rs10896027rs7119750rs11820062 was not the influencing factor of ovarian epithelial carcinoma. The haplotype of RelA gene was not the influencing factor of ovarian epithelial carcinoma P0.05RelA gene was not the influencing factor of ovarian epithelial carcinoma P0.05RelA gene diplotype. CC-CC is the influential factor of epithelial ovarian carcinoma (P0.05). Conclusion the relationship between NF- 魏 B ~ (1) RelA gene polymorphism and susceptibility to ovarian epithelial carcinoma in Han Chinese women is related to the CC-CC and ovarian epithelia of GA-GARelA and rs1182002002 CTs of the two genes (rs3774934GAand rs3774938A). Cancer is associated with susceptibility.
【作者单位】：福建省厦门市妇幼保健院林巧稚妇儿医院妇科;北京大学医学部病理教研室;北京大学第三医院妇科;
【分类号】：R737.31

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