妊娠早期外周血白细胞线粒体DNA含量与妊娠糖尿病患病率的关系

发布时间：2018-02-20 04:17

本文关键词： 妊娠并发症线粒体DNA含量妊娠糖尿病妊娠早期　出处：《山东医药》2015年20期 　论文类型：期刊论文

【摘要】：目的观察妊娠早期外周血白细胞线粒体DNA(mt DNA)含量是否与妊娠糖尿病(GDM)患病相关。方法选取妊娠12孕周者206例,应用实时定量PCR法检测研究对象外周血妊娠早期白细胞mt DNA含量的值,以mt DNA与核DNA(n DNA)含量比值(mt DNA/n DNA)表示;根据mt DNA含量中位数分为低mt DNA含量组(n=103)和高mt DNA含量组(n=103)。测定妊娠早期空腹葡萄糖、空腹胰岛素等葡萄糖代谢指标,计算胰岛素抵抗指数(HOMA-IR);妊娠24~28周行口服葡萄糖耐量试验,分别统计两组GDM患病率。结果低mt DNA含量组和高mt DNA含量组妊娠24~28周GDM患病率分别为10.7%和6.8%,P0.05;两组妊娠早期空腹葡萄糖、胰岛素和HOMA-IR比较,P均0.05。结论妊娠早期外周血白细胞mt DNA含量与GDM无明显相关性。
[Abstract]:Objective to investigate whether the mitochondrial DNA(mt DNA content in peripheral blood leukocytes in early pregnancy is related to the prevalence of gestational diabetes mellitus (GDM). Methods 206 cases of gestational 12 gestational weeks were selected. A real-time quantitative PCR method was used to detect the mt DNA content in peripheral white blood leukocytes in early pregnancy period, which was expressed by the ratio of mt DNA to nuclear DNA(n DNA (mt DNA/n DNA). According to the median content of Mt DNA, they were divided into low Mt DNA group and high Mt DNA group. Fasting glucose and fasting insulin were measured in early pregnancy, insulin resistance index (HOMA-IRN) was calculated, and oral glucose tolerance test was performed at 2428 weeks of gestation. Results the prevalence rate of GDM in the low MT DNA group and the high Mt DNA group was 10.7% and 6.8% P0.05 respectively, the fasting glucose in the first trimester of pregnancy was significantly higher than that in the control group, and the prevalence rate of GDM in the low MT DNA group and the high MT DNA group was 10.7% and 6.8% P0.05respectively. Conclusion there is no significant correlation between the level of MT DNA in peripheral blood leukocytes and GDM in early pregnancy.
【作者单位】：广州医科大学附属第二医院;南方医科大学第三附属医院;广东省妇幼保健院;广州医科大学附属第三医院;Alington
【基金】：广东省广州市科技计划项目(12C22021649)
【分类号】：R714.256

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