负调控因子鼠双微体2基因的单核苷酸多态性309与子宫内膜腺癌的相关性研究

发布时间：2018-02-23 02:01

本文关键词： 单核苷酸多态性子宫内膜癌负调控因子鼠双微体　出处：《中国临床药理学杂志》2017年15期 　论文类型：期刊论文

【摘要】：目的观察负调控因子鼠双微体2(MDM2)基因的单核苷酸(SNP)309多态性与子宫内膜样腺癌易感性及淋巴结转移的相关性。方法入选在我院手术的子宫内膜样腺癌患者187例为试验组,以同期参与常规体格检查的40岁以上健康妇女200例作为对照组。用DNA抽提试剂盒从研究对象的外周血标本中提取DNA,用限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术测定MDM2-SNP309单核苷酸多态基因型。结果试验组的MDM2-SNP309 G等位基因频率为61.76%,对照组为48.75%,差异有统计学意义(P0.05);其中试验组GG基因型频率为39.57%,对照组为19.50%,差异有统计学意义(P0.01)。试验组GG基因型发生淋巴结转移的概率为28.38%,TG型为8.43%,TT型为6.67%,差异有统计学意义(GG vs TG,P0.01;GG vs TT,P0.05)。结论 MDM2基因SNP309纯合子GG基因型是子宫内膜样腺癌发生的遗传易感因素,也是发生淋巴结转移的危险因素。
[Abstract]:Objective to investigate the association between SNP309 polymorphism of the double microbody 2MDM2) gene and susceptibility to endometrial adenocarcinoma and lymph node metastasis. Methods 187 patients with endometrioid adenocarcinoma operated in our hospital were selected as the trial group. Two hundred healthy women over 40 years old who participated in routine physical examination at the same time were used as control group. DNA extraction kit was used to extract DNA from peripheral blood samples of the study subjects, and PCR-RFLP technique was used to detect DNA by restriction fragment length polymorphism polymerase chain reaction (RFLP). Results the frequency of MDM2-SNP309 G allele was 61.76 in the trial group and 48.75 in the control group, the difference was statistically significant (P 0.05), and the frequency of the GG genotype in the test group was 39.57 and that in the control group was 19.50. The difference was statistically significant (P 0.01). In the trial group, the probability of lymph node metastasis of GG genotype was 28.38 and that of TG was 8.43, and that of TT was 6.67. The difference was statistically significant. Conclusion MDM2 gene SNP309 homozygous GG genotype is a genetic susceptibility factor for endometrial adenocarcinoma. It is also a risk factor for lymph node metastasis.
【作者单位】：电子科技大学附属医院四川省人民医院妇产科;
【基金】：四川省卫生厅科学研究基金资助项目(080383)
【分类号】：R737.33

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