维生素E对妊娠期糖尿病小鼠氧化应激及炎症因子的影响

发布时间：2018-02-25 04:28

本文关键词： 维生素E 妊娠期糖尿病氧化应激炎症　出处：《卫生研究》2017年03期 　论文类型：期刊论文

【摘要】：目的研究维生素E对妊娠期糖尿病小鼠氧化应激反应及炎症因子的影响。方法将C57BL/6J孕鼠随机分为3组:空白对照组、妊娠期糖尿病(GDM)模型组、维生素E干预组,模型组和干预组腹腔注射0.25%链脲佐菌素(STZ)60 mg/kg造模,随后干预组灌胃维生素E 5 mg/(kg·d),其余两组给予等量生理盐水,连续7天,定时监测体重及血糖,实验结束后处死小鼠,检测血清氧化应激指标谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、丙二醛(MDA)和炎症因子TNF-α、IL-6、IL-8水平。结果维生素E可上调GDM小鼠胰岛素水平(P0.05),但未改善血糖水平,提高SOD和GSH-Px活性、降低MDA水平(P0.05),降低TNF-α、IL-6、IL-8水平(P0.05)。结论维生素E可通过改变GDM小鼠体内的氧化应激内环境和降低炎症水平来改善葡萄糖调节能力和胰岛素水平。
[Abstract]:Objective to study the effects of vitamin E on oxidative stress and inflammatory factors in gestational diabetic mice. Methods pregnant mice were randomly divided into three groups: blank control group, GDM model group and vitamin E intervention group. The model group and the intervention group were injected intraperitoneally with 0.25% streptozotocin (STZ) for 60 mg/kg, then the intervention group was given intragastric administration of vitamin E 5 mg/(kg 路dl, and the other two groups were given the same amount of normal saline for 7 days. The mice were regularly monitored for body weight and blood glucose, and the mice were killed after the experiment. The levels of GSH-PxD, SOD, MDA, TNF- 伪, IL-6 and IL-8 in serum were measured. Results Vitamin E upregulated the insulin level of GDM mice (P 0.05), but did not improve the blood glucose level and the activities of SOD and GSH-Px. Conclusion Vitamin E can improve glucose regulation ability and insulin level by changing the internal environment of oxidative stress and reducing the level of inflammation in GDM mice.
【作者单位】：安徽医科大学公共卫生学院营养与食品卫生学系;
【基金】：国家自然科学基金(No.81373011)
【分类号】：R714.256

【相似文献】