IL-24联合顺铂对宫颈癌移植瘤侵袭和转移能力的影响

发布时间：2018-02-28 01:25

本文关键词： IL-24 顺铂宫颈癌侵袭转移　出处：《山西医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：研究IL-24基因联合顺铂对宫颈癌裸鼠移植瘤的生长抑制作用以及对肿瘤细胞侵袭、转移能力的影响。方法：将构建成功的宫颈癌裸鼠模型随机分为6组：PBS组（瘤体内注射磷酸盐缓冲液）、pDC316空质粒组（瘤体内注射脂质体包裹的空质粒pDC316）、IL-24组（瘤体内注射脂质体包裹的基因重组质粒pDC316-hIL-24）、半量顺铂组（腹腔内注射顺铂2.5mg/kg）、全量顺铂组（腹腔内注射顺铂5mg/kg）、联合组（瘤体内注射pDC316-hIL-24联合腹腔内注射半量顺铂），通过比较各组移植瘤体积和动物体重变化，评价IL-24基因联合顺铂的抑瘤效果和药物副作用，应用PCR法测定移植瘤中hIL-24的表达，westernblot法检测各组移植瘤组织中β-catenin和E-cadherin的表达变化，免疫组化方法研究各组淋巴结转移情况。结果：人宫颈癌Siha细胞裸鼠模型建立成功；PCR结果表明hIL-24成功转染入肿瘤细胞并表达；联合组治疗后移植瘤体积与全量顺铂组治疗后肿瘤体积差别无统计学意义（P＞0.05）；联合组裸鼠体重明显大于全量顺铂组（P＜0.05）；联合组下调β-catenin和上调E-cadherin的表达效果最明显，，显微镜下观察见联合组淋巴结中肿瘤细胞数较少且有部分坏死。结论：IL-24基因联合半量顺铂治疗能达到全量顺铂的抑瘤效果，减少化疗药物的使用剂量；IL-24基因可通过下调β-catenin和上调E-cadherin的表达水平进而抑制Siha肿瘤细胞侵袭和转移；联合治疗比单独使用IL-24或顺铂能更大程度抑制Siha细胞侵袭和转移。
[Abstract]:Aim: to study the effects of IL-24 gene combined with cisplatin on the growth inhibition and invasion and metastasis of cervical carcinoma xenografts in nude mice. Methods: a successful nude mouse model of cervical cancer was randomly divided into 6 groups: 1: PBS group (pDC316 empty plasmid group was injected with phosphate buffer solution in the tumor (pDC316) empty plasmid pDC316- IL-24 was injected into the tumor body (liposome encapsulated group was injected into the tumor body). The recombinant plasmid pDC316-hIL-24, half dose cisplatin group (intraperitoneal injection of cisplatin 2.5 mg / kg), whole dose group (intraperitoneal injection of cisplatin 5 mg / kg), combined group (intratumoral injection of pDC316-hIL-24 and intraperitoneal injection of half dose of cisplatin) were used to compare the volume of transplanted tumor and the weight of animals. To evaluate the anti-tumor effect and side effect of IL-24 combined with cisplatin, the expression of hIL-24 in transplanted tumor was detected by PCR method and the expression of 尾 -catenin and E-cadherin was detected by Western blot. The lymph node metastasis in each group was studied by immunohistochemical method. Results: human cervical cancer Siha cells were successfully transfected into tumor cells and expressed in nude mice. There was no significant difference in tumor volume between the combined group and the whole cisplatin group (P > 0.05), the weight of nude mice in the combined group was significantly higher than that in the whole cisplatin group (P < 0.05), the effect of down-regulating 尾 -catenin and up-regulation of E-cadherin was the most obvious in the combined group. It was observed under microscope that the number of tumor cells in the combined group was small and there was partial necrosis. Conclusion the tumor inhibition effect of total cisplatin can be achieved by the combination of 1: IL-24 gene and half dose cisplatin. Reducing the dosage of chemotherapeutic drugs can inhibit the invasion and metastasis of Siha tumor cells by down-regulating the expression of 尾 -catenin and up-regulating the expression of E-cadherin. Combination therapy can significantly inhibit the invasion and metastasis of Siha cells compared with IL-24 or cisplatin alone.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

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