CD147在不明原因自然流产患者绒毛组织的表达

发布时间：2018-02-28 14:02

本文关键词： 不明原因自然流产 CD 滋养细胞细胞增殖细胞侵袭　出处：《中国病理生理杂志》2016年11期 　论文类型：期刊论文

【摘要】：目的:分析CD147在不明原因自然流产患者绒毛组织中的表达水平,并探讨其生物学功能。方法:收集人早孕期正常和不明原因自然流产妇女的绒毛组织,采用免疫组织化学、RT-PCR和Western blotting法检测CD147的表达,比较CD147在正常早孕与不明原因自然流产妇女绒毛组织的表达差异。体外分离纯化获得正常人早孕期滋养细胞,采用免疫细胞化学验证CD147在滋养细胞的表达,引入抗CD147中和性抗体处理人滋养细胞,采用Brd U增殖实验和Transwell侵袭实验分析滋养细胞增殖和侵袭能力的变化。结果:正常早孕期绒毛组织和滋养细胞高表达CD147,不明原因自然流产绒毛组织CD147的mRNA和蛋白表达水平均低于正常早孕期绒毛组织。抗CD147中和性抗体处理原代人滋养细胞后,滋养细胞的增殖能力未发生明显变化,侵袭能力下降。结论:人绒毛组织CD147的异常低表达可能与不明原因早期妊娠失败相关,CD147通过调控滋养细胞的侵袭能力参与正常妊娠的维持。
[Abstract]:Objective: To explore the expression level of CD147 in the villi of patients with unexplained spontaneous abortion, and to investigate its biological function. Methods: normal early pregnancy and Unexplained Spontaneous Abortion villus tissues using immunohistochemistry to detect the expression of CD147 RT-PCR and Western blotting method, the difference in expression of villi of normal early pregnancy and Unexplained Spontaneous abortion. CD147 isolated from normal human first trimester trophoblast cells and purified in vitro expression by immunocytochemistry verified CD147 in trophoblast cells, the introduction of anti CD147 neutralizing antibody treatment of human trophoblast cells, the changes of invasion and proliferation ability of U proliferation nourish Brd assay and Transwell invasion assay. Results: the high expression of CD147 in normal villi tissues and trophoblast cells, mRNA protein and Unexplained Spontaneous Abortion villus tissue CD147 expression levels were Lower than normal villi tissue. Anti CD147 neutralizing antibody in primary cultured human trophoblast cells, trophoblast cell proliferation did not change significantly, the invasion ability decreased. Conclusion: the abnormal low expression of human chorionic tissue CD147 may be associated with unexplained early pregnancy failure, the CD147 is involved in the regulation of trophoblast invasion in normal pregnancy to maintain.

【作者单位】：同济大学附属第一妇婴保健院;复旦大学附属妇产科医院;
【基金】：国家自然科学基金资助项目(No.81300505) 国家卫生和计划生育委员会妇幼司公益性行业科研专项卫生科研专项资助项目(No.201402004)
【分类号】：R714.21

【相似文献】