硫化氢在外源性SB203580预处理人卵巢癌SKOV3细胞增殖中的作用

发布时间：2018-02-28 16:19

本文关键词： 卵巢癌细胞硫化氢 p丝裂原活化蛋白激酶(MAPK)信号通路细胞增殖　出处：《中国老年学杂志》2017年17期 　论文类型：期刊论文

【摘要】：目的探讨硫化氢(H2S)供体硫氢化钠(Na HS)对人卵巢癌SKOV3细胞增殖的影响及可能机制。方法体外培养SKOV3细胞,随机分为正常对照组、Na HS组、SB203580组、Na HS+SB203580组,采用四甲基偶氮唑蓝(MTT)法检测各组SKOV3细胞增殖,流式细胞术检测各组SKOV3细胞周期分布,Western印迹检测各组SKOV3细胞中P-p38MAPK蛋白表达。结果与正常对照组比较,Na HS组SKOV3细胞增殖明显增加,G1期细胞明显减少,S期细胞明显增加,且p-p38MAPK表达明显增加(均P0.01);SB203580组、Na HS+SB203580组SKOV3细胞增殖均明显减少,G1期细胞均明显增加,S期细胞均明显减少,且p-p38MAPK表达均明显降低(均P0.01)。而与Na HS组比较,SB203580组、Na HS+SB203580组SKOV3细胞增殖均明显减少,G1期细胞均明显增加,S期细胞均明显减少,且p-p38MAPK表达均明显降低(均P0.01);与SB203580组比较,Na HS+SB203580组SKOV3细胞的增殖明显增加,G1期细胞明显减少,S期细胞明显增加,且p-p38MAPK表达明显增加(均P0.01)。结论 H2S可能通过活化p38MAPK信号通路促进SKOV3细胞增殖。
[Abstract]:Objective to investigate the effect of sodium sulphide (H2S) donor sodium sulphide on the proliferation of human ovarian cancer SKOV3 cells and its possible mechanism. Methods SKOV3 cells were cultured in vitro and randomly divided into two groups: normal control group (NHS group), SB203580 group (n = 10) and Na-HS SB203580 group (n = 10). The proliferation of SKOV3 cells in each group was detected by MTT assay. The expression of P-p38 MAPK protein in SKOV3 cells was detected by flow cytometry. Results compared with the normal control group, the proliferation of SKOV3 cells in Na-HS group increased significantly, and the cells in G _ 1 phase decreased significantly. Moreover, the expression of p-p38 MAPK was significantly increased (P 0.01 / SB203580 group). The proliferation of SKOV3 cells in the Na HS SB203580 group was significantly decreased, and the cells in G 1 phase were significantly increased and the S phase cells were significantly decreased. The expression of p-p38 MAPK was significantly decreased (P0.01), while the proliferation of SKOV3 cells in SB203580 group was significantly decreased compared with that in NaHS SB203580 group. The expression of p-p38 MAPK was significantly decreased (P 0.01), and the proliferation of SKOV3 cells in the Na HS SB203580 group was significantly increased compared with that in the SB203580 group. The expression of p-p38 MAPK was significantly increased (P 0.01). Conclusion H2S may promote the proliferation of SKOV3 cells by activating p38 MAPK signaling pathway.
【作者单位】：石河子大学医学院第一附属医院妇产科;
【基金】：国家重大攻关专项(No.2013GS650104)
【分类号】：R737.31

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