量子点多重染色在卵巢癌体外成像的应用

发布时间：2018-03-02 19:10

本文选题：量子点　切入点：多重染色　出处：《中国老年学杂志》2015年17期 　论文类型：期刊论文

【摘要】：目的利用量子点(QDs)免疫荧光技术对卵巢癌SKOV3细胞上叶酸受体(FR)及人附睾蛋白(HE)4进行定位,初步揭示QDs多重染色在卵巢癌细胞体外成像的应用价值。方法采用活泼酯法,构建不同发射波长的QDs-链霉亲和素(SA)复合物,分别与生物素化-叶酸(FA)及生物素化-抗HE4抗体结合,形成特异性荧光探针,同时靶向结合SKOV3细胞,实现细胞双重染色。结果构建的QD-FA及QD-抗HE4抗体靶向探针具有极强的特异性,量子点多重染色的荧光强度与单重染色相比差异无统计学意义(P0.05)。量子点具有极强的光学稳定性。结论量子点多重染色在卵巢癌细胞体外成像中具有一定的应用价值,可为卵巢癌早期检测提供一种新思路。
[Abstract]:Objective to localize folic acid receptor (FRR) and human epididymal protein (HE4) on ovarian cancer SKOV3 cells by using quantum dot QDs- immunofluorescence technique, and to reveal the value of QDs multiple staining in imaging ovarian cancer cells in vitro. Methods active ester method was used. QDs- streptavidin (SA) complexes with different emission wavelengths were constructed, which were combined with biotinylated folate (FAA) and biotinylated anti HE4 antibodies to form specific fluorescent probes and target SKOV3 cells. Results the QD-FA and QD- anti HE4 antibody targeting probes were highly specific. There is no significant difference in fluorescence intensity between QDs and QDs compared with single staining. Quantum Dots have strong optical stability. Conclusion Quantum Dot Multiple-staining is valuable in the imaging of ovarian cancer cells in vitro. It may provide a new idea for early detection of ovarian cancer.
【作者单位】：南昌大学第二附属医院;南昌大学食品科学与技术国家重点实验室;
【基金】：国家自然科学基金项目(No.81201691) 江西省教育厅基金资助项目(GJJ3093)
【分类号】：R737.31

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