妊娠滋养细胞肿瘤患者的NLRP7蛋白caspase-1蛋白及IL-1β因子表达研究

发布时间：2018-03-04 02:25

本文选题：妊娠滋养细胞肿瘤　切入点：葡萄胎　出处：《浙江大学》2014年硕士论文　论文类型：学位论文

【摘要】：背景通过对家族性复发性葡萄胎的研究发现NLRP7基因是家族性复发性葡萄胎致病基因,可能通过炎症通路导致其发生。葡萄胎是良性妊娠滋养细胞肿瘤,葡萄胎来源的妊娠滋养细胞肿瘤是否与NLRP7基因相关尚不确定。研究目的本研究通过对妊娠滋养细胞肿瘤患者和正常对照妇女的外周血淋巴细胞培养,检测其NLRP7蛋白,caspase-1蛋白,pro-IL-1β蛋白和IL-1β细胞因子的表达情况,间接检测葡萄胎来源的妊娠滋养细胞肿瘤患者是否会引起功NLRP7蛋白功能障碍导致Casepase-1依赖的IL-1β因子的分泌异常。材料与方法选取10例病例组,10例对照组,其中病例组：10例GTT(先期妊娠为葡萄胎妊娠,且化疗前抽血)对照组：10例正常人群(纳入标准：没有SLE等自身免疫性疾病的,没有不良妊娠史的,没有炎症的人群)。分别对患者组和对照组经行LPS刺激,收集培养上液经行IL-1β因子测定,收集细胞采用Western Blotting方法测定NLRP7蛋白及caspase-1蛋白,pro-IL-1β蛋白表达情况,并对2组进行比较。结果 (1)IL-1β细胞因子的分泌：通过分析IL-1β细胞因子的分泌数据可得,LPS刺激后,病例组IL-1β细胞因子的分泌略低于对照组。2对病例差异明显(标号11,,55)。 (2)NLRP7蛋白及caspase-1蛋白表达：通过western blotting对6对NLRP7蛋白及caspase-1蛋白结果可得：2组NLRP7蛋白表达无差异,但其中2对病例48kd casepase-1蛋白在病例组无表达(标号11,,55)。 (3) pro-IL-1β蛋白表达：通过western blotting2对pro-IL-1β蛋白表达结果可得：病例组pro-IL-1β蛋白表达略高于对照组。结论：葡萄胎来源的妊娠滋养细胞肿瘤48kd casepase-1在病例组不能正常表达,可能是casepase-1依赖的IL-1β细胞因子分泌低的可能原因之一,IL-1β细胞因子低分泌可能是葡萄胎来源的妊娠滋养细胞肿瘤的发病原因之一。
[Abstract]:Background. Through the study of familial recurrent hydatidiform mole, NLRP7 gene is found to be a gene causing familial recurrent hydatidiform mole, which may be caused by inflammation pathway. Hydatidiform mole is a benign gestational trophoblastic tumor. It is unclear whether gestational trophoblastic tumors derived from hydatidiform mole are associated with NLRP7 gene. Research purpose. In this study, the expression of NLRP7 protein caspase-1 protein pro-IL-1 尾 and IL-1 尾 cytokines in peripheral blood lymphocytes of patients with gestational trophoblastic tumor and normal women were detected. Indirect detection of gestational trophoblastic neoplasms from hydatidiform mole may lead to dysfunction of NLRP7 protein and abnormal secretion of Casepase-1 dependent IL-1 尾 factor. Materials and methods. Ten cases of control group were selected from 10 cases of case group, of which 10 cases in case group: 10 cases of GTT (gestation of hydatidiform mole before chemotherapy), 10 cases of normal population (inclusion standard: no autoimmune disease such as SLE, no history of adverse pregnancy), and 10 cases of normal population (inclusion standard: no autoimmune disease such as SLE, no history of adverse pregnancy). LPS stimulation was performed in the patients group and the control group, and the IL-1 尾 factor was measured in the culture supernatant. The expression of NLRP7 protein and caspase-1 protein pro-IL-1 尾 protein were measured by Western Blotting method, and the comparison was made between the two groups. Results. The secretion of IL-1 尾 cytokines:. By analyzing the secretion data of IL-1 尾 cytokines, it was found that the secretion of IL-1 尾 cytokines in the case group was slightly lower than that in the control group. The expression of NLRP7 protein and caspase-1 protein:. There was no difference in the expression of NLRP7 protein between the two groups by western blotting in 6 pairs of NLRP7 protein and caspase-1 protein, but no expression of casepase-1 protein on 48kd protein was found in 2 pairs of cases. 3) expression of pro-IL-1 尾 protein:. The expression of pro-IL-1 尾 protein in the case group was slightly higher than that in the control group through the expression of pro-IL-1 尾 protein by western blotting2. Conclusion:. Gestational trophoblastic tumors derived from hydatidiform mole were not expressed normally in 48 kd casepase-1. It may be one of the possible reasons for the low secretion of IL-1 尾 cytokines dependent on casepase-1. The low secretion of IL-1 尾 cytokines may be one of the causes of gestational trophoblastic tumors derived from hydatidiform mole.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

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