多囊卵巢综合征痰湿证患者血清代谢组学分析及其生物标志物筛选

发布时间：2018-03-04 14:12

本文选题：多囊卵巢综合征　切入点：代谢组学　出处：《山东医药》2015年37期 　论文类型：期刊论文

【摘要】：目的应用基于核磁共振的代谢组学方法研究多囊卵巢综合征(PCOS)痰湿证患者和健康人的血清代谢物的差异,探讨与PCOS痰湿证相关的生物标志物及其发病机制。方法对收集到的PCOS痰湿证患者、健康人临床血清样本进行核磁共振氢谱预处理,随后使用SIMCA-P+软件进行模式识别多变量分析。经主成分分析法、偏最小二乘法-判别分析方法分析数据后,再经正交偏最小二乘法-判别分析进行修正,通过分析组间的差异代谢成分,最终筛选鉴定出PCOS痰湿证的生物标志物。结果 PCOS痰湿证患者和健康人之间的血清代谢成分存在差异。PCOS痰湿证患者较健康人血清中降低的代谢物有1-甲基组氨酸、柠檬酸、乙醇、甘油磷酸胆碱、低密度脂蛋白L1、脂质L6(P0.05),增加的有乙酰乙酸、极低密度脂蛋白L5、脂质L7、乳酸、赖氨酸、N-乙酰糖蛋白、苏氨酸(P0.05)。结论上述13种代谢物可能是PCOS痰湿证潜在的生物标志物。PCOS痰湿证患者血清代谢组分的变化机制和三羧酸循环、糖代谢、脂代谢和氨基酸代谢密切相关,主要表现为三羧酸循环障碍、糖代谢缓慢、脂肪动员加强。
[Abstract]:Objective to study the difference of serum metabolites between patients with phlegm-dampness syndrome and healthy persons with polycystic ovary syndrome (PCOS) by using the method of metabolomics based on nuclear magnetic resonance (MRI). To explore the biomarkers related to phlegm and dampness syndrome of PCOS and its pathogenesis. Methods the clinical serum samples of PCOS patients with phlegm dampness syndrome and healthy people were pretreated with nuclear magnetic resonance spectroscopy (NMR). After analyzing the data by principal component analysis, partial least squares and discriminant analysis, the data were corrected by orthogonal partial least squares and discriminant analysis. By analyzing the different metabolic components among the groups, Finally, the biomarkers of phlegm and dampness syndrome of PCOS were identified. Results there were differences in serum metabolites between patients with phlegm and dampness syndrome of PCOS and healthy persons. There were 1-methylhistidine and citric acid in serum of PCOS patients with phlegm dampness syndrome. Ethanol, glycerophosphate choline, low density lipoprotein L1, lipid L6, P0. 05, increased acetoacetic acid, very low density lipoprotein L5, lipid L7, lactic acid, lysine N acetyl glycoprotein, Conclusion the 13 metabolites mentioned above may be the potential biomarker of phlegm and dampness syndrome of PCOS. The change mechanism of serum metabolic components and the metabolism of tricarboxylic acid, glucose, lipid and amino acid are closely related to the changes of serum metabolic components in patients with phlegm and dampness syndrome of PCOS. The main performance is the disorder of tricarboxylic acid cycle, slow glucose metabolism and enhanced fat mobilization.
【作者单位】：山东中医药大学;
【基金】：国家自然科学基金资助项目(81273667);国家自然科学基金青年科学基金资助项目(81102560)
【分类号】：R711.75

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