量子点免疫荧光原位杂交法检测宫颈癌组织芯片中高危型HPV的感染状况

发布时间：2018-03-07 01:22

本文选题：宫颈癌　切入点：组织芯片　出处：《广东医学》2017年15期 　论文类型：期刊论文

【摘要】：目的研究宫颈癌及相关组织高危型HPV的感染状况,并评价所用检测方法的特异性和敏感性。方法回顾性采集124例石蜡包埋宫颈(癌)组织,包括80例宫颈鳞状细胞癌,24例宫颈腺癌,以及20例因子宫肌瘤或子宫腺肌病等原因切除的正常宫颈组织或炎性宫颈组织。收集宫颈癌实验标本及建立宫颈癌临床病理信息库;标本分组并按要求制作切片(含白片、HE切片及组织芯片制作)。最后应用量子点免疫荧光原位杂交技术(QDs-FISH)检测高危型HPV。结果 QDs-FISH结合biotin-labeled DNA探针检测宫颈(癌)组织,显示HrHPV阳性信号位于肿瘤细胞胞核。在正常宫颈组织中未见Hr-HPV阳性表达;80例宫颈鳞状细胞癌细胞有67例阳性表达,占83.75%;24例腺癌标本有18例阳性表达,占66.66%。Hr-HPV仅在癌组织中高表达,与正常宫颈组织相比,差异有统计学意义(P0.05);而鳞状细胞癌与腺癌组织中的表达差异无统计学意义(P0.05)。结论 (1)Hr-HPV感染与宫颈癌的发生发展密切相关。(2)QDs-FISH方法取材方便,依从性好,有较高的敏感性和特异性,重复性好,检测设备也易于操作,可作为筛查宫颈癌的一种较好方法。
[Abstract]:Objective to study the infection status of high-risk HPV in cervical cancer and related tissues, and to evaluate the specificity and sensitivity of the methods used. Methods 124 cases of paraffin-embedded cervical tissues were collected retrospectively. Including 80 cases of cervical squamous cell carcinoma 24 cases of cervical adenocarcinoma and 20 cases of normal cervix tissues or inflammatory cervix tissues resected due to uterine leiomyoma or adenomyosis etc. The cervical cancer specimens were collected and the clinical pathological information bank of cervical cancer was established. The specimens were divided into groups and made into sections (including HE sections and tissue chips) according to the requirements. Finally, high risk HPVs were detected by quantum dot immunofluorescence in situ hybridization (QDs-FISHH). Results QDs-FISH and biotin-labeled DNA probes were used to detect cervical (carcinoma) tissues. There were 67 cases of positive expression of HrHPV in 80 cases of cervical squamous cell carcinoma, 18 cases of positive expression in 24 cases of adenocarcinoma, and 66.66% of Hr-HPV was only overexpressed in cancer tissue, which showed that the positive signal of HrHPV was located in the nucleus of the tumor cell, and there were 67 cases of positive expression of Hr-HPV in 80 cases of cervical squamous cell carcinoma, and 18 cases of positive expression of Hr-HPV in 24 cases of adenocarcinoma. Compared with normal cervical tissues, the difference was statistically significant (P 0.05), but there was no significant difference in the expression of Hr-HPV between squamous cell carcinoma and adenocarcinoma. Conclusion the infection of Hr-HPV is closely related to the occurrence and development of cervical cancer. It has high sensitivity and specificity, good reproducibility and easy to operate, so it can be used as a better method for screening cervical cancer.
【作者单位】：哈励逊国际和平医院妇产科;衡水市第五人民医院妇产科;衡水市第二人民医院妇产科;哈励逊国际和平医院生殖医学科;
【分类号】：R737.33

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