ILK-ASODN对卵巢癌HO-8910细胞增殖及凋亡影响的研究

发布时间：2018-03-07 02:21

本文选题：ILK-ASODN　切入点：卵巢癌　出处：《现代妇产科进展》2015年01期 　论文类型：期刊论文

【摘要】：目的:探讨脂质体介导的整合素连接激酶(ILK)反义寡核苷酸(ASODN)对卵巢癌细胞的增殖及诱导其凋亡的作用。方法:选用ILK的反义寡核苷酸(ILKASODN)、正义寡核苷酸(ILK-SODN)分别转染卵巢癌HO-8910细胞株。实验分为6组:空白对照组(A组)、脂质体对照组(B组)、ILK-SODN 100nmol/L组(C组)和ILK-ASODN60nmol/L、80nmol/L、100nmol/L组(分别为D、E、F组)。应用四唑单钠盐(WST-1)法检测转染后第24h、48h、72h、96h和120h的体外增殖抑制率;转染120h后,采用Annexin VFITC和碘化丙啶(PI)联合标记法测定细胞凋亡情况。结果:(1)与对照组比较,转染ILK-ASODN后,卵巢癌细胞生长受到明显抑制(P0.05),ILK-ASODN各浓度组分别于96h、72h、48h开始出现明显抑制作用,且抑制率随转染浓度的增高而增高;转染ILKSODN组的细胞生长也受到抑制(P0.05),但抑制效果低于ILK-ASODN各组。(2)转染ILK-ASODN后,细胞凋亡率增高(P0.05),其凋亡率随转染浓度的增高而增高;转染ILK-SODN后细胞凋亡率也有所增高,但低于转染ILK-ASODN组(P0.05)。结论:ILKASODN可能通过诱导细胞凋亡来实现抑制卵巢癌细胞的生长效应,从而最终达到抑制肿瘤发展、转移和恶化的目的。
[Abstract]:Objective: to investigate the effect of liposome-mediated integrin ligand kinase (ILK) antisense oligonucleotide (ASODN) on proliferation and apoptosis of ovarian cancer cells. Methods: ILK antisense oligodeoxynucleotides (ILK) were used to transfect ILK-SODN. Ovarian cancer HO-8910 cell lines were divided into 6 groups: control group A, liposome control group B, ILK-SODN 100nmol / L group C) and ILK-ASODN 60nmol / L ~ (80nmol / L) / L ~ (100) nmol 路L ~ (-1) group respectively. After 120 hours of transfection, apoptosis was determined by Annexin VFITC and Pi labeling. Results compared with the control group, the growth of ovarian cancer cells was significantly inhibited after transfection of ILK-ASODN. The growth of ovarian cancer cells was inhibited by P0.05 and ILK-ASODN at 96 h, 72 h and 48 h after transfection, and the inhibition began to appear at 96 h, 72 h and 48 h, respectively. The inhibition rate increased with the increase of transfection concentration, and the cell growth in ILKSODN group was also inhibited by P0.05G, but the inhibitory effect was lower than that in ILK-ASODN group (P 0.05), and the apoptosis rate increased with the increase of transfection concentration. After transfection of ILK-SODN, the cell apoptosis rate was also increased, but lower than that in the transfected ILK-ASODN group (P 0.05). Conclusion\\\% ILKASODN may inhibit the growth of ovarian cancer cells by inducing apoptosis, and finally achieve the purpose of inhibiting tumor development, metastasis and deterioration.
【作者单位】：哈尔滨医科大学附属第二医院妇产科;哈尔滨医科大学附属第三院妇科;
【基金】：黑龙江省教育厅面上项目资助(No:11511158)
【分类号】：R737.31

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