FOXC2在宫颈癌组织中的表达及其临床意义

发布时间：2018-03-10 01:40

  本文选题：FOXC2　切入点：宫颈癌　出处：《吉林大学》2014年硕士论文　论文类型：学位论文

【摘要】：全世界每年大约有50万宫颈癌新发病例，成为女性生殖系统最常见的三大恶性肿瘤之一。宫颈癌的治愈率低，5年存活率仅为3.2%～13.0%[1]。每年大约有25万妇女死于宫颈癌，占肿瘤总死亡病例的8%［2］。虽然广泛开展的宫颈癌普查工作已有效降低了其病死率，但近20年来，宫颈癌发病率在逐年增高，且呈年轻化趋势，国内外已均有青春期少女患宫颈癌的病例报道。人乳头状瘤病毒（HPV)感染作为宫颈癌发生的主要病因已被大多数人所知（以16、18型最常见），但HPV感染的患者中仅有一小部分最终发展成浸润性宫颈癌，我们推测可能存在其他因素促进宫颈癌发生发展。宫颈癌的转移途径主要为直接蔓延和淋巴结转移，且早期即可发生淋巴结转移，癌细胞经宫旁转移至盆腔各组淋巴结再至腹主动脉旁淋巴结，最终至全身性转移。由于宫颈癌早期常无明显临床症状、现有的筛查方法检出率尚不稳定，所以明确宫颈癌的发生、发展、侵袭、转移的分子生物学基础，找到特异性强、灵敏度高的肿瘤标记物，对宫颈癌的早期诊断、早期治疗和判断预后具有重要价值。 人叉头框-C2（Forkhead Box C2，FOXC2）是一种人胚胎蛋白，有证据显示它在胚胎心血管、淋巴管、软骨组织、骨骼、肾脏、主动脉弓及眼睛间质等许多部位表达。胚胎和肿瘤组织在细胞向远处迁移等某些生物学行为上，表现出极大的相似性。近年来亦有研究表明，，FOXC2参与了乳腺癌、淋巴瘤、子宫腺肌症及妇科恶性肿瘤的侵袭和转移，但报道FOXC2蛋白在宫颈癌中的检测相对较少，值得进一步研究。本实验收集了2010年1月-2013年4月于吉林大学第一医院接受手术治疗的正常宫颈组织21例，CIN组织30例（其中CINI-II组11例、CINIII组19例），宫颈癌组织58例。应用免疫组化方法检测FOXC2在正常宫颈组织、宫颈上皮内瘤变（cervical intraepithelial neoplasia，CIN）和宫颈癌组织中的表达情况，研究FOXC2在宫颈癌发生、发展、侵袭和转移中的作用，分析FOXC2与宫颈癌各临床病理特征之间的关系，如临床期别、分化程度、有无淋巴结转移、年龄及肿物大小等。探讨FOXC2在宫颈癌的早期诊断、监测复发和判断预后中的临床意义。
[Abstract]:There are about 500 thousand new cases of cervical cancer each year in the world, has become one of the three most common malignant tumor of the female reproductive system of cervical cancer. The cure rate is low, the 5 year survival rate is only 3.2% ~ 13.0%[1]. every year there are about 250 thousand women died of cervical cancer, total cancer deaths accounted for 8% of 2. Although the screening of cervical the work has been carried out a wide range of cancer can reduce the mortality rate, but in the past 20 years, the incidence of cervical cancer increased year by year, and the trend was younger, both at home and abroad have reported cases of adolescent girls suffering from cervical cancer. Human papillomavirus (HPV) infection as the main cause of cervical cancer has been most people know (the most common type of 16,18), but HPV infection in patients with only a small part of eventually develop into invasive cervical cancer, we speculate that there may be other factors to promote the development of cervical cancer. Cervical cancer is the main way to transfer straight The spread of metastasis and lymph nodes, and occurs in the early stage of lymph node metastasis, cancer cells by parametrial lymph node metastasis to the pelvic and paraaortic lymph nodes, ultimately to systemic metastasis. As a result of early cervical cancer have no obvious clinical symptoms, screening methods of existing detection rate is not stable, so clear of cervical cancer the occurrence, development, invasion and metastasis, molecular biology, found strong specificity and high sensitivity of tumor markers in the early diagnosis of cervical cancer, has important value for early treatment and prognosis.
Forkhead box -C2 (Forkhead Box C2, FOXC2) is a human embryo protein, there is evidence of it in embryonic heart, lymphatic vessels, cartilage, bone, kidney, the expression of aortic arch and interstitial eyes and many other parts. The embryo and the tumor tissue to the distant migration of some biological behavior in cells. Show great similarity. Recently, studies have shown that FOXC2 is involved in breast cancer, lymphoma, adenomyosis and gynecological malignant tumor invasion and metastasis, but reported the detection of FOXC2 protein in cervical cancer is relatively small, it is worth further research. The normal cervical tissue were collected during January 2010 -2013 April in No.1 Hospital of Jilin University surgical treatment of 21 cases, 30 cases of CIN tissues (including 11 cases of CINI-II group, CINIII group, 19 cases), 58 cases of cervical carcinoma. Immunohistochemical method was used to detect FOXC2 in normal cervical tissues, cervical intraepithelial neoplasia ( Cervical intraepithelial neoplasia, CIN) expression and cervical carcinoma, FOXC2 in cervical cancer occurrence, development, invasion and metastasis, to analyze the relationship between FOXC2 and cervical cancer clinical pathological features, such as clinical stage, degree of differentiation, lymph node metastasis, age and tumor size. Study of FOXC2 in the early diagnosis of cervical cancer and the clinical significance of monitoring the recurrence and prognosis.

【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R737.33

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