siRNA沉默EZH2基因对人宫颈癌细胞侵袭转移的影响

发布时间：2018-03-11 04:22

本文选题：EZH基因　切入点：宫颈癌　出处：《西安交通大学学报(医学版)》2017年05期 　论文类型：期刊论文

【摘要】：目的探讨靶向沉默EZH2基因对宫颈癌细胞侵袭转移的影响及其分子机制。方法采用化学合成siRNA瞬时转染人宫颈癌C33A细胞,沉默EZH2基因的表达。通过细胞划痕实验、Transwell小室侵袭实验、软琼脂克隆形成实验,观察沉默EZH2对宫颈癌细胞迁移、侵袭及体外成瘤能力的影响,Western blot法检测MMP2的表达变化。结果 EZH2siRNA转染C33A细胞后,细胞划痕及Transwell小室侵袭实验结果均显示宫颈癌细胞的迁移、侵袭能力明显减低,差异具有统计学意义;软琼脂克隆形成实验表明,EZH2沉默后克隆形成数目明显减少,差异具有统计学意义;Western blot结果显示MMP2表达水平下调。结论沉默EZH2基因表达能显著抑制宫颈癌C33A细胞的侵袭转移能力,其作用机制可能通过下调MMP2来实现。
[Abstract]:Objective to investigate the effect of targeted silencing EZH2 gene on invasion and metastasis of cervical cancer cells and its molecular mechanism. Methods the expression of EZH2 gene was instantly transfected into human cervical cancer C33A cells by chemically synthesized siRNA. The effect of silencing EZH2 on the migration, invasion and tumorigenic ability of cervical cancer cells in vitro was observed. The expression of MMP2 was detected by Western blot. Results after transfection of EZH2siRNA into C33A cells, the expression of MMP2 was detected by Western blot. The results of cell scratch and Transwell chamber invasion showed that the migration and invasion ability of cervical cancer cells were significantly decreased, the difference was statistically significant, the results of soft Agar clone formation test showed that the number of colony formation was significantly decreased after EZH2 silencing. Conclusion silencing the expression of EZH2 gene can significantly inhibit the invasion and metastasis of cervical cancer C33A cells, and its mechanism may be achieved by down-regulation of MMP2.
【作者单位】：西安交通大学第一附属医院妇产科;
【基金】：陕西省国际科技合作与交流计划项目(No.2013KW-27-01)~~
【分类号】：R737.33

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