乳胞素联合卡铂对卵巢癌SKOV3细胞的增殖抑制作用和促凋亡作用

发布时间：2018-03-11 13:37

本文选题：卵巢肿瘤　切入点：SKOV细胞　出处：《吉林大学学报(医学版)》2017年06期 　论文类型：期刊论文

【摘要】：目的:探讨蛋白酶体抑制剂乳胞素(LAC)对体外卵巢癌SKOV3细胞增殖和凋亡的影响,并阐明其作用机制。方法:体外培养卵巢癌SKOV3细胞,采用0、2.5、5.0、10.0和20.0μmol·L~(-1) LAC干预SKOV3细胞48h,5μmol·L~(-1) LAC干预SKOV3细胞0、24、48和72h;将细胞分为对照组(细胞不经药物干预)、LAC组(加入5μmol·L~(-1) LAC)、卡铂组(加入10、20、40及80μmol·L~(-1)卡铂)和LAC联合卡铂组(加入5μmol·L~(-1) LAC和10、20、40、80μmol·L~(-1)卡铂)。采用MTT法和流式细胞术(FCM)检测各组卵巢癌SKOV3细胞的增殖抑制率和凋亡率。结果:MTT检测,随着LAC作用时间的延长和浓度的升高,SKOV3细胞的增殖受到明显抑制,48h时LAC的半数抑制浓度(IC50)为5.36μmol·L~(-1);LAC联合卡铂组细胞的增殖抑制率较相同浓度卡铂组明显升高(P0.05),卡铂的IC50由58.08μmol·L~(-1)下降至18.37μmol·L~(-1)。FCM检测,随着LAC作用时间的延长,SKOV3细胞凋亡率呈升高趋势;与卡铂组和LAC组比较,LAC联合卡铂组SKOV3细胞凋亡率明显升高(P0.05)。结论:LAC可有效抑制卵巢癌SKOV3细胞的体外增殖且能诱导其凋亡,并可以明显增强卡铂对卵巢癌SKOV3细胞株的增殖抑制作用。
[Abstract]:Objective: to investigate the effects of lactobacillus inhibitor (Lactadine) on proliferation and apoptosis of ovarian cancer SKOV3 cells in vitro and to elucidate its mechanism. Methods: ovarian cancer SKOV3 cells were cultured in vitro. #number0# 渭 mol 路L ~ (-1) LAC was used to treat SKOV3 cells for 48 h and 5 渭 mol 路L ~ (-1) LAC) LAC was used to treat SKOV3 cells for 48 h and 72 h, respectively, and the cells were divided into two groups: control group (cells without drug intervention) with LACU (5 渭 mol 路L ~ (-1)), carboplatin group (10 渭 mol 路L ~ (-1)) and LAC combined with carboplatin group (5 渭 mol 路L ~ (-1)) LAC and capriplatin group (added 5 渭 mol 路L ~ (-1)) and capplatin group (add 10 渭 mol 路L ~ (-1)) and LAC combined with carboplatin group (5 渭 mol 路L ~ (-1)) and 5 渭 mol 路L ~ (-1) LAC. The proliferation inhibition rate and apoptosis rate of ovarian cancer SKOV3 cells in each group were detected by MTT and flow cytometry. When the proliferation of SKOV3 cells was significantly inhibited with the prolongation of the time and concentration of LAC, the IC50 of LAC was 5.36 渭 mol 路L ~ (-1) and carboplatin was 5.36 渭 mol 路L ~ (-1) + carboplatin. The proliferation inhibition rate of SKOV3 cells was significantly higher than that of the same concentration of carboplatin. IC50 decreased from 58.08 渭 mol 路L ~ (-1) to 18.37 渭 mol 路L ~ (-1) ~ (-1). Compared with carboplatin group and LAC group, the apoptosis rate of SKOV3 cells increased significantly with the prolongation of LAC. Conclusion: LAC can effectively inhibit the proliferation of ovarian cancer SKOV3 cells in vitro and induce apoptosis. And the inhibitory effect of carboplatin on the proliferation of ovarian cancer SKOV3 cell line was significantly enhanced.
【作者单位】：吉林大学中日联谊医院妇产科;
【基金】：吉林省财政厅科研基金资助课题(sczsy201523)
【分类号】：R737.31

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