PIAS-1基因真核表达质粒的构建与鉴定

发布时间：2018-03-11 20:47

  本文选题：宫颈癌　切入点：PIAS-　出处：《实用临床医药杂志》2014年16期 　论文类型：期刊论文

【摘要】：目的构建PIAS-1 siRNA干扰载体Plko.1、PIAS-1。方法设计以PIAS-1基因为靶标的siRNA序列,并克隆人载体Plko.1,用基因测序进行重组克隆验证。结果 PCR检测证实siRNA插入Plko.1质粒,测序分析证实插入序列正确。结论成功构建PIAS-1基因的siRNA载体,为研究PIAS-1基因对宫颈癌细胞增殖凋亡的影响提供了稳定转染的骨架载体。
[Abstract]:Objective to construct PIAS-1 siRNA interference vector Plko.1PIAS-1.Methods A siRNA sequence targeting PIAS-1 was designed and cloned into human vector Plko.1.Recombinant cloning was performed by gene sequencing. Results PCR analysis confirmed that siRNA was inserted into Plko.1 plasmid. Conclusion the siRNA vector of PIAS-1 gene was constructed successfully, which provides a stable transfection skeleton vector for studying the effect of PIAS-1 gene on the proliferation and apoptosis of cervical cancer cells.
【作者单位】： 兰州大学第一临床学院妇科;甘肃省人民医院妇科;
【基金】：国家自然科学基金(81260387)
【分类号】：R737.33;Q78
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本文编号：1599839