人早孕滋养细胞卵泡抑素表达水平与自发流产的关系

发布时间：2018-03-11 21:43

本文选题：卵泡抑素　切入点：激活素A　出处：《吉林大学》2017年硕士论文　论文类型：学位论文

【摘要】：自发流产在所有临床确认的妊娠中,大约占15%,其中孕早期流产约为80%。流产是指妊娠未满28周、胎儿体重未满1000g而使妊娠终止,早期流产为发生在妊娠12周以前者。诱发自然流产的病因极其复杂,常见原因有:免疫因素、遗传缺陷、感染性疾病、内分泌紊乱、生殖道解剖因素等,这些因素可能同时参与导致流产的发生。但仍有部分患者发病诱因不明,有研究显示这部分自发流产患者中有40%~80%可能为免疫因素所致。自发流产不仅对女性患者及家庭带来众多困扰,其伴发生殖器官并发症,严重者会导致大出血而危及生命。卵泡抑素(Follistatin,FS)是激活素(Activin)结合蛋白,Activin A是转化生长因子(TGF-β)超家族成员,FS可以中和该家族成员的生物学功能而发挥其相应的作用。有研究发现,妊娠期间FS及Activin A主要来源于母胎界面,即蜕膜和绒毛组织。本课题组前期检测的小样本结果发现正常孕早期血清FS的表达水平高于自发流产患者,然而自发流产患者Activin A水平变化,以及血清FS降低是否能够真正作为预测自发流产的辅助诊断指标,目前尚缺乏深入研究。因此,本次研究首先通过进一步扩大临床检测样本,确定绒毛滋养细胞FS及Activin A的表达与自发流产之间的关系;其次,体外培养人绒毛滋养细胞系HTR-8/SVneo细胞,探索FS对该细胞的生物学功能的影响,通过滋养细胞的增殖及分泌活性的分析,进一步揭示FS参与自发流产的作用机制,对于辅助诊断临床自发流产,积极进行有效治疗,改善患者生活质量都具有十分重要的意义。
[Abstract]:Spontaneous abortion accounts for about 15% of all clinically confirmed pregnancies, of which the early stage of pregnancy is about 80%. Abortion refers to the termination of pregnancy within 28 weeks of gestation and less than 1000g of fetal weight. The cause of induced spontaneous abortion is extremely complicated. The common causes are immune factors, genetic defects, infectious diseases, endocrine disorders, anatomical factors of reproductive tract, and so on. These factors may also be involved in the occurrence of miscarriage. Studies have shown that 40% of the patients with spontaneous abortion may be caused by immune factors. Spontaneous abortion not only brings many troubles to female patients and families, but also complicates the complications of reproductive organs. In severe cases, it can cause massive bleeding and endanger life. Follicle inhibitor FollistatinFSis activin Activin-binding protein Activin A is the transforming growth factor TGF- 尾) superfamily member FFS can neutralize the biological function of this family member and play its corresponding role. FS and Activin A during pregnancy were mainly derived from the maternal and fetal interface, i.e. decidual and chorionic villi. The results of small samples in the early stage of pregnancy showed that the expression of FS in the normal early pregnancy was higher than that in the spontaneous abortion. However, the changes of Activin A level in spontaneous abortion patients and whether the decrease of serum FS can really be used as an auxiliary diagnostic index to predict spontaneous abortion is still lack of in-depth study. To determine the relationship between the expression of FS and Activin A in villus trophoblast cells and spontaneous abortion. Secondly, to explore the effect of FS on the biological function of human villus trophoblastic cell line HTR-8/SVneo cells in vitro. Through the analysis of the proliferation and secretory activity of trophoblast, the mechanism of FS involved in spontaneous abortion is further revealed, which is of great significance for the diagnosis of spontaneous abortion, the effective treatment and the improvement of patients' quality of life.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R714.21

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