TGR5和NF-κB在妊娠期肝内胆汁淤积症患者胎盘组织中的表达及意义

发布时间：2018-03-12 19:15

本文选题：妊娠期肝内胆汁淤积症　切入点：胎盘巨噬细胞　出处：《重庆医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的:检测ICP患者胎盘组织中TGR5和NF-κB的表达，初步探讨TGR5和NF-κB在ICP中的作用。方法:选取2013年3月-11月在重庆医科大学附属第一医院产科行剖宫产的39例ICP孕妇为实验对象，其中，，21例为ICP轻度组,18例为ICP重度组。对照组为同期行择期剖宫产术的30例正常孕妇。应用免疫组化方法检测各组孕妇胎盘组织TGR5和NF-κB的表达情况。结果:(1)TGR5、NF-κB均表达于孕妇胎盘巨噬细胞及滋养细胞细胞膜和细胞质中。（2）在胎盘巨噬细胞中，ICP组TGR5表达强度低于对照组（Z=-4.237，P0.05)，ICP重度组TGR5表达强度低于轻度组（Z=-2.351，P0.05）；病例组NF-κB表达强度高于对照组（Z=-2.266，P0.05)；ICP重度组NF-κB表达强度高于轻度组（Z=-3.017，P0.05）。（3）在胎盘滋养细胞中，病例组TGR5表达强度低于对照组（Z=-2.397，P0.05)，ICP重度组TGR5表达强度低于轻度组（Z=-3.207，P0.05）；病例组NF-κB表达强度高于对照组（Z=-3.105，P0.05)；ICP重度组NF-κB表达强度高于轻度组（Z=-2.671，P0.05）。（4）TGR5和NF-κB在ICP组胎盘巨噬细胞中表达行相关性分析，呈负相关,相关系数分别为:-0.584，P值小于0.05；TGR5和NF-κB在ICP组胎盘滋养细胞中表达关系，也呈负相关,相关系数为:-0.424,P值小于0.05。结论:ICP胎盘巨噬细胞和滋养细胞中TGR5表达下调，NF-κB表达上调，推测TGR5—NF-κB途径可能参与ICP病理生理过程。
[Abstract]:Aim: to detect the expression of TGR5 and NF- 魏 B in placenta of patients with ICP and to explore the role of TGR5 and NF- 魏 B in ICP. Methods: from March 2013 to November, 39 pregnant women with ICP underwent cesarean section in the first affiliated Hospital of Chongqing Medical University. The expression of TGR5 and NF- 魏 B in placenta of 21 cases of mild ICP and 18 cases of severe ICP and 30 cases of normal pregnant women undergoing elective cesarean section at the same time were detected by immunohistochemical method. Results the TGR5 expression intensity of TGR5 in placental macrophages and trophoblast cell membrane and cytoplasm of pregnant women was lower than that in the control group (P 0.05, P 0.05), and the expression intensity of TGR5 in the severe group was lower than that in the mild group, and that in the case group was significantly lower than that in the mild group (P < 0.05), and the expression of TGR5- 魏 B in the placental macrophages was significantly lower than that in the control group (P < 0.05), while the expression of TGR5 in the placental macrophages was significantly lower than that in the control group (P 0.05). The expression of NF- 魏 B in the severe ICP group was higher than that in the mild group, and the expression of NF- 魏 B in the placental trophoblastic cells was higher than that in the mild group. The expression intensity of TGR5 in the severe group was lower than that in the mild group. The expression of NF- 魏 B in the severe group was higher than that in the control group. The expression intensity of NF- 魏 B in the severe group was higher than that in the mild group. The correlation between NF- 魏 B and NF- 魏 B in the placental macrophages of the ICP group was also analyzed, and the correlation between the expression of NF- 魏 B and the expression of NF- 魏 B in the placental macrophages of the mild group was also analyzed, and the correlation between the expression of NF- 魏 B and the expression of NF- 魏 B in the placental macrophages of the mild group was also analyzed, and the correlation between the expression of NF- 魏 B and the expression of NF- 魏 B in the placental macrophages was analyzed. There was a negative correlation between the expression of TGR5 and NF- 魏 B in placental trophoblastic cells in ICP group, and the correlation coefficient was: 1 / -0. 584 (P < 0. 05). Conclusion the down-regulation of TGR5 expression in ICP placental macrophages and trophoblastic cells suggests that TGR5-NF- 魏 B pathway may be involved in the pathophysiological process of ICP.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R714.25

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